PF3D7_0623400 (Mei2)

Genome location: Pf3D7_06_v3:950,223..951,506(-)

Genome classification: Core

Function and Localization

Product Description: MEI2-like RNA-binding protein

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:1990904	ribonucleoprotein complex
Function	GO:0003723	RNA binding
Process	GO:0045836	positive regulation of meiotic nuclear division
Process	GO:0020014	schizogony

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	possibly expressed	0.09	0.94
Schizont	possibly expressed	0.02	0.98
Gametocyte	expressed	0.19	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0623400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.41 | MFS: -3.192 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1122300)

Relative Growth Rate: 1.05 ± 0.14
Confidence: 5.31

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1122300)

Modification: Disrupted | RMgm-4941

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KT70

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103687

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	13	13	28
disruptive	3	20	14	32
missense	3	20	14	31

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	42	13	33
disruptive	6	54	43	93
missense	6	50	38	73

PlasmoDB Total SNPs: 18

Non-coding: 0 | Synonymous: 10 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 427 | Molecular Weight (kDa): 49.93

UniProt ID(s): C6KT70

PDB ID(s): None

Isoelectric Point: 8.05

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007201	Mei2/Mei2-like, C-terminal RNA recognition motif
InterPro	IPR035979	RNA-binding domain superfamily
PFam	PF04059	Mei2/Mei2-like, C-terminal RNA recognition motif
Superfamily	SSF54928	RNA-binding domain superfamily

Associated Publications

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
26883588	A Plasmodium yoelii Mei2-Like RNA Binding Protein Is Essential for Completion ofLiver Stage Schizogony.	Dankwa DA, Davis MJ, Kappe SHI, Vaughan AM	10.1128/IAI.01417-15
32484795	A replication-competent late liver stage-attenuated human malaria parasite	Goswami D, Betz W, ..., Kappe SH	10.1172/jci.insight.135589
30257894	mmi1 and rep2 mRNAs are novel RNA targets of the Mei2 RNA-binding protein duringearly meiosis in Schizosaccharomyces pombe	Mukherjee K, Futcher B, Leatherwood J	10.1098/rsob.180110
37887293	Thirty Years with E RH: An m RNA Splicing and Mitosis Factor Only or Rather a Novel Genome Integrity Protector?	Kozlowski P	10.3390/cells12202449
31533287	meiRNA, A Polyvalent Player in Fission Yeast Meiosis	Yamashita A	10.3390/ncrna5030045
37788281	A dominant negative 14-3-3 mutant in Schizosaccharomyces pombe distinguishes thebinding proteins involved in sexual differentiation and check point	Ohshima T, Jiajun Z, ..., Kawamukai M	10.1371/journal.pone.0291524
36333336	Creation and preclinical evaluation of genetically attenuated malaria parasitesarresting growth late in the liver	Franke-Fayard B, Marin-Mogollon C, ..., Janse CJ	10.1038/s41541-022-00558-x