Product Description: ribonuclease H2 subunit A, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.26.4 (Ribonuclease H)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0032299 | ribonuclease H2 complex |
| Function | GO:0004523 | RNA-DNA hybrid ribonuclease activity |
| Process | GO:0043137 | DNA replication, removal of RNA primer |
| Process | GO:0006401 | RNA catabolic process |
| Process | GO:0090502 | RNA phosphodiester bond hydrolysis, endonucleolytic |
| Process | GO:0006298 | mismatch repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.26 | 0.87 |
| Trophozoite | expressed | 0.31 | 0.80 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | expressed | 0.48 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0623900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.315 | MFS: -2.412 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1122800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1122800)
Modification: Disrupted | RMgm-3054
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT75
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.26.4 | ribonuclease H | KClNEMSDSDTTNaFDNAEDTAPCMBNH4ClmtSSBDextranmanicol... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100555
Most Similar Human Ortholog: O75792
TM-align score: 0.89 | RMSD: 1.64
Seq Identity: 0.46 | Length: 267 / 288
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000104889 | ribonuclease H2 subunit A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 21 | 9 | 18 |
| disruptive | 4 | 12 | 9 | 23 |
| missense | 4 | 12 | 9 | 19 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 37 | 15 | 13 |
| disruptive | 5 | 43 | 20 | 53 |
| missense | 4 | 34 | 18 | 33 |
PlasmoDB Total SNPs: 44
Non-coding: 29 | Synonymous: 11 | Nonsynonymous: 3 | Stop Codon: 1
Protein Length: 288 | Molecular Weight (kDa): 33.095
UniProt ID(s): C6KT75
PDB ID(s): None
Isoelectric Point: 6.96
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001352 | Ribonuclease HII/HIII |
| InterPro | IPR004649 | Ribonuclease H2, subunit A |
| InterPro | IPR012337 | Ribonuclease H-like superfamily |
| InterPro | IPR024567 | Ribonuclease HII/HIII domain |
| PFam | PF01351 | Ribonuclease HII/HIII domain |
| Superfamily | SSF53098 | Ribonuclease H-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |