Product Description: hexokinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.- (Phosphotransferases with an alcohol group as acceptor.);2.7.1.1 (Hexokinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0036396 | RNA N6-methyladenosine methyltransferase complex |
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0008865 | fructokinase activity |
| Function | GO:0004340 | glucokinase activity |
| Function | GO:0004396 | hexokinase activity |
| Function | GO:0019158 | mannokinase activity |
| Process | GO:0001678 | cellular glucose homeostasis |
| Process | GO:0051156 | glucose 6-phosphate metabolic process |
| Process | GO:0006096 | glycolytic process |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.32 | 0.84 |
| Trophozoite | expressed | 0.66 | 0.63 |
| Schizont | expressed | 0.20 | 0.85 |
| Gametocyte | expressed | 0.30 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0624000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.462 | MFS: -2.565 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1122900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q02155
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.36) with 3O1W (Global RMSD=4.68)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q38C42 | Phosphotransferase | Trypanosoma brucei | OMA, OrthoDB, OrthoMCL | Glucosamine derivative, 6Glucosamine derivative, 8 |
| P52792 | Hexokinase-4 | OMA, OrthoDB | US9409900, 6US9409900, 13US9409900, 65US9409900, 78... | |
| Q38C41 | Phosphotransferase | Trypanosoma brucei | OrthoDB, OrthoMCL | med.21724, Compound 153MLS000055225lipoic acid (LA)MLS000550500... |
| P35557 | Hexokinase-4 | Homo sapiens | OrthoDB | benzamide derivative, 6benzamide derivative, 13hbenzamide derivative, 13lbenzamide derivative, 13q... |
| Q2TB90 | Hexokinase HKDC1 [W721R] | Homo sapiens | OrthoDB | MLS000084034cid_68261MLS000052721prostaglandin F2 methyl ester... |
| P52789 | Hexokinase-2 | OrthoDB | CHEMBL3805703CHEMBL3805460CHEMBL3804930CHEMBL3805765... | |
| P17712 | Hexokinase-4 | Rattus norvegicus | OrthoDB | CHEMBL178370CHEMBL178308CHEMBL359618CHEMBL360155... |
| P19367 | Hexokinase-1 | Homo sapiens | OrthoDB | MLS000084626MLS000339491MLS000681605MLS001002736... |
Ortholog Group (OrthoMCL): OG6_100418
Most Similar Human Ortholog: E9PB90
TM-align score: 0.84 | RMSD: 2.24
Seq Identity: 0.31 | Length: 440 / 493
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000156510 | hexokinase domain containing 1 |
| ENSG00000156515 | hexokinase 1 |
| ENSG00000159399 | hexokinase 2 |
| ENSG00000160883 | hexokinase 3 |
Protein Length: 493 | Molecular Weight (kDa): 55.261
PDB ID(s): None
Isoelectric Point: 7.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001312 | Hexokinase |
| InterPro | IPR019807 | Hexokinase, binding site |
| InterPro | IPR022672 | Hexokinase, N-terminal |
| InterPro | IPR022673 | Hexokinase, C-terminal |
| InterPro | IPR043129 | ATPase, nucleotide binding domain |
| PFam | PF00349 | Hexokinase, N-terminal |
| PFam | PF03727 | Hexokinase, C-terminal |
| Superfamily | SSF53067 | ATPase, nucleotide binding domain |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 31 | 7 | 30 |
| disruptive | 1 | 6 | 3 | 13 |
| missense | 1 | 6 | 3 | 9 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 34 | 17 | 25 |
| disruptive | 2 | 18 | 13 | 43 |
| missense | 1 | 12 | 10 | 28 |
PlasmoDB Total SNPs: 134
Non-coding: 127 | Synonymous: 6 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 23954294 | Studies with the Plasmodium falciparum hexokinase reveal that PfHT limits therate of glucose entry into glycolysis. | Tjhin ET, Staines HM, ..., Saliba KJ | 10.1016/j.febslet.2013.07.052 |
| 27458230 | Identification of Novel Plasmodium falciparum Hexokinase Inhibitors withAntiparasitic Activity. | Davis MI, Patrick SL, ..., Morris JC | 10.1128/AAC.00914-16 |
| 31384004 | Transcriptome-wide dynamics of extensive m(6)A mRNA methylation during Plasmodiumfalciparum blood-stage development. | Baumgarten S, Bryant JM, ..., Scherf A | 10.1038/s41564-019-0521-7 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 37628920 | Structural Analysis of Plasmodium falciparum Hexokinase Provides Novel Information about Catalysis Due to a Plasmodium-Specific Insertion | Dillenberger M, Werner AD, ..., Fritz-Wolf K | 10.3390/ijms241612739 |
| 38423908 | Potential inhibitory effect of Auricularia auricula polysaccharide on advancedglycation end-products (AGEs) | Gong P, Pei S, ..., Guo Y | 10.1016/j.ijbiomac.2024.129856 |