Product Description: ISWI chromatin-remodeling complex ATPase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.- (Acting on ATP; involved in cellular and subcellular movement.);3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0008094 | ATP-dependent activity, acting on DNA |
| Function | GO:0003677 | DNA binding |
| Function | GO:0003723 | RNA binding |
| Function | GO:0004386 | helicase activity |
| Function | GO:0000976 | transcription cis-regulatory region binding |
| Process | GO:0006338 | chromatin remodeling |
| Process | GO:0045893 | positive regulation of transcription, DNA-templated |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 2.40 | 0.17 |
| Trophozoite | expressed | 2.39 | 0.12 |
| Schizont | expressed | 2.65 | 0.11 |
| Gametocyte | expressed | 1.98 | 0.22 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0624600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.992 | MFS: -2.746 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1123500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 164 | 72 | 185 |
| disruptive | 107 | 228 | 133 | 326 |
| missense | 33 | 152 | 102 | 227 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 62 | 308 | 120 | 145 |
| disruptive | 187 | 515 | 282 | 647 |
| missense | 69 | 333 | 187 | 370 |
PlasmoDB Total SNPs: 454
Non-coding: 208 | Synonymous: 131 | Nonsynonymous: 114 | Stop Codon: 1
Protein Length: 2719 | Molecular Weight (kDa): 315.62
UniProt ID(s): C6KT82
PDB ID(s): None
Isoelectric Point: 6.96
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000330 | SNF2-related, N-terminal domain |
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR001841 | Zinc finger, RING-type |
| InterPro | IPR001965 | Zinc finger, PHD-type |
| InterPro | IPR011011 | Zinc finger, FYVE/PHD-type |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR019786 | Zinc finger, PHD-type, conserved site |
| InterPro | IPR019787 | Zinc finger, PHD-finger |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00176 | SNF2-related, N-terminal domain |
| PFam | PF00271 | Helicase, C-terminal |
| PFam | PF00628 | Zinc finger, PHD-finger |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF57903 | Zinc finger, FYVE/PHD-type |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19765590 | Potential epigenetic regulatory proteins localise to distinct nuclearsub-compartments in Plasmodium falciparum. | Volz J, Carvalho TG, ..., Cowman AF | 10.1016/j.ijpara.2009.09.002 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 32816370 | Exploring the virulence gene interactome with CRISPR/dCas9 in the human malariaparasite. | Bryant JM, Baumgarten S, ..., Scherf A | 10.15252/msb.20209569 |