Genome location: Pf3D7_06_v3:1,008,978..1,010,796(-)
Genome classification: Core
Product Description: N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 3.5.1.89 (N-acetylglucosaminylphosphatidylinositol deacetylase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0000225 | N-acetylglucosaminylphosphatidylinositol deacetylase activity |
| Function | GO:0016811 | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| Process | GO:0006506 | GPI anchor biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.03 | 0.98 |
| Trophozoite | expressed | 0.11 | 0.92 |
| Schizont | expressed | 0.07 | 0.95 |
| Gametocyte | expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0624700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.99 | MFS: -2.574 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1123600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT83
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.5.1.89 | N-acetylglucosaminylphosphatidylinositol deacetylase | EDTAethambutolmetaraminolN-hydroxybenzamide2-hydroxybenzoic acidsalicylic hydroxamic acid4-bromo-N-hydroxybenzamide4-bromo-N,2-dihydroxybenzamide5-benzamido-N,2-dihydroxybenzamideN-hydroxy-N'-(4-methoxyphenyl)urea5-amino-N-(benzyloxy)-2-hydroxybenzamide2-(carboxymethyl)-2-deoxy-D-glucopyranose... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101964
Most Similar Human Ortholog: Q9Y2B2
TM-align score: 0.91 | RMSD: 1.71
Seq Identity: 0.29 | Length: 241 / 247
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000108474 | phosphatidylinositol glycan anchor biosynthesis class L |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 14 | 7 | 18 |
| disruptive | 3 | 26 | 14 | 37 |
| missense | 3 | 24 | 13 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 27 | 10 | 17 |
| disruptive | 15 | 59 | 28 | 63 |
| missense | 10 | 50 | 24 | 45 |
PlasmoDB Total SNPs: 114
Non-coding: 98 | Synonymous: 11 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 247 | Molecular Weight (kDa): 30.058
UniProt ID(s): C6KT83
PDB ID(s): None
Isoelectric Point: 9.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003737 | N-acetylglucosaminyl phosphatidylinositol deacetylase-related |
| InterPro | IPR024078 | Putative deacetylase LmbE-like domain superfamily |
| InterPro | IPR039516 | N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase |
| PFam | PF02585 | N-acetylglucosaminyl phosphatidylinositol deacetylase-related |
| Superfamily | SSF102588 | Putative deacetylase LmbE-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 35222844 | Compounds targeting GPI biosynthesis or N-glycosylation are active againstPlasmodium falciparum | Fenollar A, Ros-Lucas A, ..., Izquierdo L | 10.1016/j.csbj.2022.01.029 |
| 34618440 | Rescue of Glycosylphosphatidylinositol-Anchored Protein Biosynthesis UsingSynthetic Glycosylphosphatidylinositol Oligosaccharides | Guerrero PA, Murakami Y, ..., Varon Silva D | 10.1021/acschembio.1c00465 |