PF3D7_0624900

Genome location: Pf3D7_06_v3:1,015,681..1,018,905(+)

Genome classification: Core

Function and Localization

Product Description: GAS8-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0005874	microtubule
Process	GO:0034613	cellular protein localization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.02	0.99
Trophozoite	no data	0.10	0.94
Schizont	no data	0.03	0.98
Gametocyte	no data	1.07	0.50

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0624900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.961 | MFS: 0.921 | #Insertions: 2

PlasmoGEM Phenotype: Insufficient data (Pb ortholog: PBANKA_1123800)

Relative Growth Rate: 0.56 ± 1.04
Confidence: 1.31

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KT85

"Best" AlphaFill ligand hit: 1CY (1-(4-chlorophenyl)-6,6-dimethyl-1,6-dihydro-1,3,5-triazine-2,4-diamine, Local RMSD=10.45) with 3UM6 (Global RMSD=40.53)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102980

Most Similar Human Ortholog: H3BUA7

TM-align score: 0.98 | RMSD: 0.39

Seq Identity: 0.07 | Length: 60 / 445

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000141013	growth arrest specific 8

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	21	12	26
disruptive	3	34	28	71
missense	3	34	28	70

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	46	17	29
disruptive	7	88	42	113
missense	5	80	38	84

PlasmoDB Total SNPs: 214

Non-coding: 186 | Synonymous: 19 | Nonsynonymous: 8 | Stop Codon: 1

Protein Information

Protein Length: 445 | Molecular Weight (kDa): 53.879

UniProt ID(s): C6KT85

PDB ID(s): None

Isoelectric Point: 9.75

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR025593	Growth arrest-specific protein 8 domain
InterPro	IPR039308	Growth arrest-specific protein 8
PFam	PF13851	Growth arrest-specific protein 8 domain
Superfamily	N/A	N/A