PF3D7_0625000 (SMS1)

Genome location: Pf3D7_06_v3:1,019,648..1,022,452(+)

Genome classification: Core

Function and Localization

Product Description: sphingomyelin synthase 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 6

EC Numbers: 2.7.8.27 (Sphingomyelin synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0030173	integral component of Golgi membrane
Component	GO:0030176	integral component of endoplasmic reticulum membrane
Component	GO:0005887	integral component of plasma membrane
Function	GO:0047493	ceramide cholinephosphotransferase activity
Function	GO:0033188	sphingomyelin synthase activity
Process	GO:0046513	ceramide biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	nd	nd
Trophozoite	expressed	nd	nd
Schizont	expressed	nd	nd
Gametocyte	expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0625000.2

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.536 | MFS: -2.574 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1123900)

Relative Growth Rate: 1.01 ± 0.06
Confidence: 6.90

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1123900)

Modification: Disrupted | RMgm-3059

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZVB5

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.8.27	sphingomyelin synthase	lyseninceramidesphingosinetumor necrosis factor-alphatricyclodecan-9-yl-xanthogenatetricyclodecane-9-yl xanthogenate2-(benzyloxy)-N-(pyridin-2-yl)benzamide2-(benzyloxy)-N-(pyridin-3-yl)benzamidepotassium tricyclodecan-9-yl-xanthogenate2-((2-ethylbenzyl)oxy)-N-(pyridin-3-yl)benzamide2-((2-chlorobenzyl)oxy)-N-(pyridin-3-yl)benzamide2-((2-fluorobenzyl)oxy)-N-(pyridin-3-yl)benzamide...
2.7.8.27	sphingomyelin synthase	lyseninceramidesphingosinetumor necrosis factor-alphatricyclodecan-9-yl-xanthogenatetricyclodecane-9-yl xanthogenate2-(benzyloxy)-N-(pyridin-2-yl)benzamide2-(benzyloxy)-N-(pyridin-3-yl)benzamidepotassium tricyclodecan-9-yl-xanthogenate2-((2-ethylbenzyl)oxy)-N-(pyridin-3-yl)benzamide2-((2-chlorobenzyl)oxy)-N-(pyridin-3-yl)benzamide2-((2-fluorobenzyl)oxy)-N-(pyridin-3-yl)benzamide...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105973

Most Similar Human Ortholog: Q86VZ5

TM-align score: 0.57 | RMSD: 2.76

Seq Identity: 0.18 | Length: 259 / 439

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000198964	sphingomyelin synthase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	17	9	23
disruptive	2	9	12	26
missense	2	9	9	22

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	41	17	28
disruptive	11	33	32	92
missense	4	24	26	63

PlasmoDB Total SNPs: 113

Non-coding: 98 | Synonymous: 14 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 439 | Molecular Weight (kDa): 51.828

UniProt ID(s): A0A143ZVB5

PDB ID(s): None

Isoelectric Point: 8.73

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR025749	Sphingomyelin synthase-like domain
PFam	PF14360	Sphingomyelin synthase-like domain
Superfamily	N/A	N/A