PF3D7_0625100 (SMS2)

Genome location: Pf3D7_06_v3:1,023,015..1,024,214(+)

Genome classification: Core

Function and Localization

Product Description: sphingomyelin synthase 2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 6

EC Numbers: 2.7.8.27 (Sphingomyelin synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0030173	integral component of Golgi membrane
Component	GO:0030176	integral component of endoplasmic reticulum membrane
Component	GO:0005887	integral component of plasma membrane
Function	GO:0047493	ceramide cholinephosphotransferase activity
Function	GO:0033188	sphingomyelin synthase activity
Process	GO:0046513	ceramide biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.00	1.00
Trophozoite	not expressed	0.01	0.99
Schizont	possibly expressed	0.01	0.99
Gametocyte	expressed	0.35	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0625100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.728 | #Insertions: 4

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1124000)

Modification: Other | RMgm-452

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KT87

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.8.27	sphingomyelin synthase	lyseninceramidesphingosinetumor necrosis factor-alphatricyclodecan-9-yl-xanthogenatetricyclodecane-9-yl xanthogenate2-(benzyloxy)-N-(pyridin-2-yl)benzamide2-(benzyloxy)-N-(pyridin-3-yl)benzamidepotassium tricyclodecan-9-yl-xanthogenate2-((2-ethylbenzyl)oxy)-N-(pyridin-3-yl)benzamide2-((2-chlorobenzyl)oxy)-N-(pyridin-3-yl)benzamide2-((2-fluorobenzyl)oxy)-N-(pyridin-3-yl)benzamide...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_164014

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	9	15	18
disruptive	6	17	4	34
missense	6	16	4	30

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	27	20	24
disruptive	9	38	28	73
missense	8	31	23	57

PlasmoDB Total SNPs: 37

Non-coding: 0 | Synonymous: 25 | Nonsynonymous: 11 | Stop Codon: 1

Protein Information

Protein Length: 399 | Molecular Weight (kDa): 46.66

UniProt ID(s): C6KT87

PDB ID(s): None

Isoelectric Point: 8.49

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR025749	Sphingomyelin synthase-like domain
PFam	PF14360	Sphingomyelin synthase-like domain
Superfamily	N/A	N/A