Genome location: Pf3D7_06_v3:1,026,468..1,031,411(-)
Genome classification: Core
Product Description: mitochondrial DNA polymerase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.7 (DNA-directed DNA polymerase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0005737 | cytoplasm |
Component | GO:0005739 | mitochondrion |
Function | GO:0008409 | 5'-3' exonuclease activity |
Function | GO:0003677 | DNA binding |
Function | GO:0003887 | DNA-directed DNA polymerase activity |
Process | GO:0006260 | DNA replication |
Process | GO:0006261 | DNA-dependent DNA replication |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | not expressed | 0.16 | 0.92 |
Trophozoite | expressed | 1.19 | 0.44 |
Schizont | expressed | 0.81 | 0.61 |
Gametocyte | expressed | 0.44 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0625300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.144 | MFS: -2.934 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1124200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT89
"Best" AlphaFill ligand hit: DCP (2'-deoxycytidine-5'-triphosphate, Local RMSD=0.27) with 3PX0 (Global RMSD=9.83)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
2.7.7.7 | DNA-directed DNA polymerase | KClEDTANH4+dATPNH4ClddTTPMK886UracilalliinsucroseethanolAllicin... |
Orthology to BindingDB Entries:
UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
---|---|---|---|---|
O75417 | DNA polymerase theta | OrthoDB | CHEMBL5185248CHEMBL5190448CHEMBL5175626CHEMBL5188740... |
Ortholog Group (OrthoMCL): OG6_101669
Most Similar Human Ortholog: Q7Z5Q5
TM-align score: 0.50 | RMSD: 4.38
Seq Identity: 0.21 | Length: 501 / 1444
All Human Orthologs (OrthoMCL):
Gene ID | Description |
---|---|
ENSG00000130997 | DNA polymerase nu |
Protein Length: 1444 | Molecular Weight (kDa): 171.13
UniProt ID(s): C6KT89
PDB ID(s): None
Isoelectric Point: 8.47
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR001098 | DNA-directed DNA polymerase, family A, palm domain |
InterPro | IPR002298 | DNA polymerase A |
InterPro | IPR043502 | DNA/RNA polymerase superfamily |
PFam | PF00476 | DNA-directed DNA polymerase, family A, palm domain |
Superfamily | SSF56672 | DNA/RNA polymerase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 11 | 53 | 35 | 82 |
disruptive | 68 | 178 | 100 | 218 |
missense | 37 | 125 | 74 | 156 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 23 | 118 | 60 | 73 |
disruptive | 129 | 401 | 166 | 403 |
missense | 64 | 262 | 120 | 247 |
PlasmoDB Total SNPs: 271
Non-coding: 70 | Synonymous: 112 | Nonsynonymous: 89 | Stop Codon: 0
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
34730376 | Bloom Helicase Along with Recombinase Rad51 Repairs the Mitochondrial Genome ofthe Malaria Parasite. | Jha P, Gahlawat A, ..., Bhattacharyya MK | 10.1128/mSphere.00718-21 |