PF3D7_0625600 (PAP)

Genome location: Pf3D7_06_v3:1,037,558..1,042,004(+)

Genome classification: Core

Function and Localization

Product Description: poly(A) polymerase PAP, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.19 (Polynucleotide adenylyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0004652	polynucleotide adenylyltransferase activity
Process	GO:0043631	RNA polyadenylation
Process	GO:0006378	mRNA polyadenylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.33	0.83
Trophozoite	expressed	0.20	0.88
Schizont	expressed	0.10	0.93
Gametocyte	possibly expressed	0.21	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0625600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.126 | MFS: -2.835 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1124500)

Relative Growth Rate: 0.03 ± 0.25
Confidence: 4.13

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KT92

"Best" AlphaFill ligand hit: 3AT (cordycepin triphosphate, Local RMSD=0.57) with 1Q79 (Global RMSD=3.71)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.19	polynucleotide adenylyltransferase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100691

Most Similar Human Ortholog: F8WAT4

TM-align score: 0.96 | RMSD: 1.31

Seq Identity: 0.46 | Length: 233 / 631

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000090060	poly(A) polymerase alpha
ENSG00000115421	poly(A) polymerase gamma
ENSG00000218823	poly(A) polymerase beta

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	30	20	39
disruptive	4	21	13	43
missense	4	21	13	32

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	16	58	27	34
disruptive	5	63	37	109
missense	5	46	31	72

PlasmoDB Total SNPs: 212

Non-coding: 189 | Synonymous: 19 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 631 | Molecular Weight (kDa): 75.164

UniProt ID(s): C6KT92

PDB ID(s): None

Isoelectric Point: 6.17

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002934	Polymerase, nucleotidyl transferase domain
InterPro	IPR007010	Poly(A) polymerase, RNA-binding domain
InterPro	IPR007012	Poly(A) polymerase, central domain
InterPro	IPR011068	Nucleotidyltransferase, class I-like, C-terminal
InterPro	IPR014492	Poly(A) polymerase
InterPro	IPR043519	Nucleotidyltransferase superfamily
PFam	PF01909	Polymerase, nucleotidyl transferase domain
PFam	PF04926	Poly(A) polymerase, RNA-binding domain
PFam	PF04928	Poly(A) polymerase, central domain
Superfamily	SSF55003	Nucleotidyltransferase, class I-like, C-terminal
Superfamily	SSF81301	Nucleotidyltransferase superfamily
Superfamily	SSF81631