PF3D7_0626100

Genome location: Pf3D7_06_v3:1,055,904..1,058,812(+)

Genome classification: Core

Function and Localization

Product Description: oxidoreductase, short-chain dehydrogenase family, putative

SignalP Peptide: N/A

# Transmembrane Domains: 3

EC Numbers: 1.1.1.36 (Acetoacetyl-CoA reductase);1.8.4.1 (Glutathione--homocystine transhydrogenase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016491	oxidoreductase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.02	0.99
Trophozoite	expressed	0.15	0.90
Schizont	expressed	0.04	0.97
Gametocyte	expressed	0.15	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0626100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.31 | MFS: -2.764 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1124900)

Relative Growth Rate: 0.46 ± 0.08
Confidence: 6.42

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1124900)

Modification: Disrupted | RMgm-3065

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KT97

"Best" AlphaFill ligand hit: AAE (acetoacetic acid, Local RMSD=0.07) with 6ZZQ (Global RMSD=2.33)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.1.1.36	acetoacetyl-CoA reductase	NAD+NADPHNADP+acetyl-CoAmalonyl-CoAbutyryl-CoAiodoacetamideacetoacetyl-CoAmethylmalonyl-CoAN-MethylmaleimideN-Phenylmaleimidesulfhydryl reagents...
1.8.4.1	glutathione-homocystine transhydrogenase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100091

Most Similar Human Ortholog: Q8TC12

TM-align score: 0.90 | RMSD: 2.41

Seq Identity: 0.28 | Length: 313 / 383

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000072042	retinol dehydrogenase 11
ENSG00000139988	retinol dehydrogenase 12
ENSG00000160439	retinol dehydrogenase 13
ENSG00000167536	dehydrogenase/reductase 13
ENSG00000186153	WW domain containing oxidoreductase
ENSG00000240857	retinol dehydrogenase 14
ENSG00000273944	retinol dehydrogenase 13
ENSG00000274418	retinol dehydrogenase 13
ENSG00000274504	retinol dehydrogenase 13
ENSG00000275474	retinol dehydrogenase 13
ENSG00000276341	retinol dehydrogenase 13
ENSG00000276684	retinol dehydrogenase 13
ENSG00000276826	retinol dehydrogenase 13
ENSG00000278149	retinol dehydrogenase 13
ENSG00000278284	retinol dehydrogenase 13

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	24	15	28
disruptive	2	18	12	40
missense	2	17	11	38

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	57	10	25
disruptive	9	62	46	106
missense	7	48	36	76

PlasmoDB Total SNPs: 196

Non-coding: 184 | Synonymous: 11 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 383 | Molecular Weight (kDa): 44.758

UniProt ID(s): C6KT97

PDB ID(s): None

Isoelectric Point: 9.68

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002347	Short-chain dehydrogenase/reductase SDR
InterPro	IPR036291	NAD(P)-binding domain superfamily
PFam	PF00106	Short-chain dehydrogenase/reductase SDR
Superfamily	SSF51735	NAD(P)-binding domain superfamily