Genome location: Pf3D7_06_v3:1,061,115..1,062,891(-)
Genome classification: Core
Product Description: 3-oxoacyl-acyl-carrier protein synthase I/II
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.1.41 (Beta-ketoacyl-[acyl-carrier-protein] synthase I)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0004315 | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| Function | GO:0004312 | fatty acid synthase activity |
| Process | GO:0006633 | fatty acid biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 1.00 |
| Trophozoite | possibly expressed | 0.03 | 0.98 |
| Schizont | possibly expressed | 0.01 | 1.00 |
| Gametocyte | expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0626300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.284 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1125100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_1126500)
Modification: Disrupted | RMgm-5370
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT99
"Best" AlphaFill ligand hit: M7U ((2r)-2-(hexadecanoyloxy)-3-{[(10r)-10-methyloctadecanoyl]oxy}propyl phosphate, Local RMSD=0.18) with 4C6W (Global RMSD=1.43)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.41 | beta-ketoacyl-[acyl-carrier-protein] synthase I | C75BABXfisetinarseniteluteolinacyl-CoAdaidzeinSB418011genisteinbaicaleinceruleninplatencin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P9WQD7 | 3-oxoacyl-[acyl-carrier-protein] synthase 2 | Mycobacterium tuberculosis | OrthoMCL, OrthoMCL BLAST | TLM analog, 1 |
| P9WQD9 | 3-oxoacyl-[acyl-carrier-protein] synthase 1 | Mycobacterium tuberculosis | OrthoMCL | CHEMBL2204380 |
| Q5TKS0 | 3-oxoacyl-[acyl-carrier-protein] synthase 2 | OrthoMCL BLAST | PlatensimycinPhenyl DihydroplatensimycinDihydroplatensimycinCHEMBL492363... |
Ortholog Group (OrthoMCL): OG6_100752
Most Similar Human Ortholog: Q9NWU1
TM-align score: 0.89 | RMSD: 1.71
Seq Identity: 0.38 | Length: 423 / 473
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000151093 | 3-oxoacyl-ACP synthase, mitochondrial |
Protein Length: 473 | Molecular Weight (kDa): 52.657
Isoelectric Point: 9.24
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000794 | Beta-ketoacyl synthase |
| InterPro | IPR014030 | Beta-ketoacyl synthase, N-terminal |
| InterPro | IPR014031 | Beta-ketoacyl synthase, C-terminal |
| InterPro | IPR016039 | Thiolase-like |
| InterPro | IPR017568 | 3-oxoacyl-[acyl-carrier-protein] synthase 2 |
| InterPro | IPR018201 | Beta-ketoacyl synthase, active site |
| InterPro | IPR020841 | Polyketide synthase, beta-ketoacyl synthase domain |
| PFam | PF00109 | Beta-ketoacyl synthase, N-terminal |
| PFam | PF02801 | Beta-ketoacyl synthase, C-terminal |
| Superfamily | SSF53901 | Thiolase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 37 | 23 | 26 |
| disruptive | 4 | 30 | 28 | 55 |
| missense | 4 | 30 | 27 | 52 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 61 | 23 | 34 |
| disruptive | 11 | 72 | 42 | 112 |
| missense | 10 | 63 | 36 | 72 |
PlasmoDB Total SNPs: 48
Non-coding: 28 | Synonymous: 14 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16467310 | Recombinant expression and biochemical characterization of the unique elongatingbeta-ketoacyl-acyl carrier protein synthase involved in fatty acid biosynthesisof Plasmodium falciparum using natural and artificial substrates. | Lack G, Homberger-Zizzari E, ..., Perozzo R | 10.1074/jbc.M509119200 |
| 19472174 | Beta-ketoacyl-ACP synthase I/II from Plasmodium falciparum (PfFabB/F)--is it B orF? | Sharma S, Sharma SK, Surolia N, Surolia A | 10.1002/iub.205 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |