Genome location: Pf3D7_06_v3:1,078,020..1,082,073(+)
Genome classification: Core
Product Description: pyruvate kinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.40 (Pyruvate kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0003723 | RNA binding |
| Function | GO:0004743 | pyruvate kinase activity |
| Process | GO:0006096 | glycolytic process |
| Process | GO:0051289 | protein homotetramerization |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.55 | 0.75 |
| Trophozoite | expressed | 1.04 | 0.48 |
| Schizont | expressed | 0.16 | 0.89 |
| Gametocyte | possibly expressed | 0.27 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0626800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.147 | MFS: -2.921 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1125600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KTA4
"Best" AlphaFill ligand hit: 144 (tris-hydroxymethyl-methyl-ammonium, Local RMSD=0.15) with 6V74 (Global RMSD=2.66)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.40 | pyruvate kinase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P30613 | Pyruvate kinase PKLR | HOGENOM | CHEMBL4128703CHEMBL4127005CHEMBL175336CHEMBL5176753... | |
| P14618 | Pyruvate kinase PKM | HOGENOM, OMA | Salicylic acid derivative, compound 2CHEMBL1939121CHEMBL1939120CHEMBL1939119... | |
| Q6GG09 | Pyruvate kinase | Staphylococcus aureus (strain MRSA252) | OMA, OrthoMCL BLAST | CHEMBL3339207CHEMBL3339206CHEMBL3339205CHEMBL3339204... |
Ortholog Group (OrthoMCL): OG6_100258
Most Similar Human Ortholog: P30613
TM-align score: 0.92 | RMSD: 1.97
Seq Identity: 0.45 | Length: 492 / 511
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000067225 | pyruvate kinase M1/2 |
| ENSG00000143627 | pyruvate kinase L/R |
| ENSG00000262785 | pyruvate kinase L/R |
Protein Length: 511 | Molecular Weight (kDa): 55.661
UniProt ID(s): C6KTA4
PDB ID(s): 3KHD, 6KSH, 7Z4M, 7Z4N, 7Z4Q, 7Z4R
Isoelectric Point: 7.52
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001697 | Pyruvate kinase |
| InterPro | IPR011037 | Pyruvate kinase-like, insert domain superfamily |
| InterPro | IPR015793 | Pyruvate kinase, barrel |
| InterPro | IPR015795 | Pyruvate kinase, C-terminal |
| InterPro | IPR015813 | Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily |
| InterPro | IPR018209 | Pyruvate kinase, active site |
| InterPro | IPR036918 | Pyruvate kinase, C-terminal domain superfamily |
| PFam | PF00224 | Pyruvate kinase, barrel |
| PFam | PF02887 | Pyruvate kinase, C-terminal |
| Superfamily | SSF50800 | Pyruvate kinase-like, insert domain superfamily |
| Superfamily | SSF51621 | Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily |
| Superfamily | SSF52935 | Pyruvate kinase, C-terminal domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 20 | 14 | 36 |
| disruptive | 1 | 3 | 1 | 15 |
| missense | 1 | 3 | 1 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 48 | 19 | 46 |
| disruptive | 2 | 12 | 21 | 95 |
| missense | 2 | 9 | 17 | 56 |
PlasmoDB Total SNPs: 157
Non-coding: 149 | Synonymous: 8 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15567170 | Functional analysis, overexpression, and kinetic characterization of pyruvatekinase from Plasmodium falciparum. | Chan M, Sim TS | 10.1016/j.bbrc.2004.11.018 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 32878705 | Pyruvate kinase from Plasmodium falciparum: Structural and kinetic insights intothe allosteric mechanism. | Zhong W, Li K, ..., Lescar J | 10.1016/j.bbrc.2020.08.048 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |