Genome location: Pf3D7_06_v3:1,099,753..1,100,995(-)
Genome classification: Core
Product Description: mitochondrial import inner membrane translocase subunit TIM22, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0042721 | TIM22 mitochondrial import inner membrane insertion complex |
| Component | GO:0005744 | TIM23 mitochondrial import inner membrane translocase complex |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0030943 | mitochondrion targeting sequence binding |
| Function | GO:0008320 | protein transmembrane transporter activity |
| Process | GO:0045039 | protein insertion into mitochondrial inner membrane |
| Process | GO:0006626 | protein targeting to mitochondrion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.01 | 0.99 |
| Trophozoite | no data | 0.07 | 0.95 |
| Schizont | no data | 0.01 | 0.99 |
| Gametocyte | no data | 0.09 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0627400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.256 | MFS: -3.331 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1126100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KTB0
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102845
Most Similar Human Ortholog: Q9Y584
TM-align score: 0.65 | RMSD: 2.73
Seq Identity: 0.28 | Length: 146 / 193
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000177370 | translocase of inner mitochondrial membrane 22 |
| ENSG00000277649 | translocase of inner mitochondrial membrane 22 |
| ENSG00000278501 | translocase of inner mitochondrial membrane 22 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 4 | 2 | 7 |
| disruptive | 1 | 6 | 8 | 28 |
| missense | 1 | 6 | 7 | 23 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 12 | 4 | 6 |
| disruptive | 3 | 35 | 16 | 41 |
| missense | 2 | 24 | 13 | 26 |
PlasmoDB Total SNPs: 49
Non-coding: 30 | Synonymous: 14 | Nonsynonymous: 4 | Stop Codon: 1
Protein Length: 193 | Molecular Weight (kDa): 21.447
UniProt ID(s): C6KTB0
PDB ID(s): None
Isoelectric Point: 9.67
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR039175 | Mitochondrial import inner membrane translocase subunit TIM22 |
| PFam | PF02466 | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 32918038 | Structure of the mitochondrial TIM22 complex from yeast | Zhang Y, Ou X, ..., Li L | 10.1038/s41422-020-00399-0 |