Genome location: Pf3D7_06_v3:1,100,997..1,103,240(-)
Genome classification: Core
Product Description: protein DJ-1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0008233 | peptidase activity |
| Function | GO:0036524 | protein deglycase activity |
| Process | GO:0106046 | guanine deglycation, glyoxal removal |
| Process | GO:0036525 | protein deglycation |
| Process | GO:0036529 | protein deglycation, glyoxal removal |
| Process | GO:0006457 | protein folding |
| Process | GO:0009228 | thiamine biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.23 | 0.89 |
| Trophozoite | no data | 0.47 | 0.73 |
| Schizont | no data | 0.04 | 0.97 |
| Gametocyte | no data | 0.23 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0627500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.297 | MFS: -2.711 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1126200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1126200)
Modification: Disrupted | RMgm-3071
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KTB1
"Best" AlphaFill ligand hit: ISN (isatin, Local RMSD=0.26) with 6AF9 (Global RMSD=2.09)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q99497 | Parkinson disease protein 7 | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | JYQ88JYQ88CHEMBL5172713CHEMBL406190... |
Ortholog Group (OrthoMCL): OG6_101257
Most Similar Human Ortholog: Q99497
TM-align score: 0.93 | RMSD: 1.11
Seq Identity: 0.35 | Length: 183 / 189
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000116288 | Parkinsonism associated deglycase |
Protein Length: 189 | Molecular Weight (kDa): 20.292
UniProt ID(s): C6KTB1
PDB ID(s): None
Isoelectric Point: 7.3
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002818 | DJ-1/PfpI |
| InterPro | IPR006287 | Protein/nucleic acid deglycase DJ-1 |
| InterPro | IPR029062 | Class I glutamine amidotransferase-like |
| PFam | PF01965 | DJ-1/PfpI |
| Superfamily | SSF52317 | Class I glutamine amidotransferase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 13 | 8 | 10 |
| disruptive | 2 | 2 | 2 | 12 |
| missense | 2 | 2 | 2 | 10 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 23 | 8 | 11 |
| disruptive | 4 | 11 | 11 | 34 |
| missense | 2 | 7 | 9 | 30 |
PlasmoDB Total SNPs: 110
Non-coding: 106 | Synonymous: 2 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25091419 | Genetic ablation of plasmoDJ1, a multi-activity enzyme, attenuates parasitevirulence and reduces oocyst production. | Singhal N, Mastan BS, Kumar KA, Sijwali PS | 10.1042/BJ20140051 |
| 27714937 | PlasmoSEP: Predicting surface-exposed proteins on the malaria parasite usingsemisupervised self-training and expert-annotated data. | El-Manzalawy Y, Munoz EE, Lindner SE, Honavar V | 10.1002/pmic.201600249 |
| 29753659 | A conserved human DJ1-subfamily motif (DJSM) is critical for anti-oxidative anddeglycase activities of Plasmodium falciparum DJ1. | Nair DN, Prasad R, ..., Sijwali PS | 10.1016/j.molbiopara.2018.05.003 |