PF3D7_0627900 (POP4)

Genome location: Pf3D7_06_v3:1,118,231..1,121,193(+)

Genome classification: Core

Function and Localization

Product Description: ribonuclease P protein subunit p29, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.26.5 (Ribonuclease P)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000172	ribonuclease MRP complex
Component	GO:0030677	ribonuclease P complex
Function	GO:0033204	ribonuclease P RNA binding
Function	GO:0004540	ribonuclease activity
Process	GO:0006364	rRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.05	0.97
Trophozoite	not expressed	0.05	0.96
Schizont	not expressed	0.03	0.98
Gametocyte	not expressed	0.06	0.96

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0627900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.657 | MFS: -3.368 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KTB5

"Best" AlphaFill ligand hit: UD1 (uridine-diphosphate-n-acetylglucosamine, Local RMSD=14.59) with 6H21 (Global RMSD=25.39)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.26.5	ribonuclease P	ATPRNAEDTANH4+tRNASO42-NeoR5NeoG6NeoK6retinolneaminemat-tRNA...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101654

Most Similar Human Ortholog: A0A0A0MQS4

TM-align score: 0.87 | RMSD: 1.71

Seq Identity: 0.29 | Length: 93 / 291

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000105171	POP4 homolog, ribonuclease P/MRP subunit

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	6	8	15
disruptive	3	17	9	24
missense	3	17	9	19

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	21	9	22
disruptive	8	44	26	77
missense	6	36	16	49

PlasmoDB Total SNPs: 131

Non-coding: 110 | Synonymous: 16 | Nonsynonymous: 5 | Stop Codon: 0

Protein Information

Protein Length: 291 | Molecular Weight (kDa): 34.318

UniProt ID(s): C6KTB5

PDB ID(s): None

Isoelectric Point: 9.63

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002730	Ribonuclease P/MRP, subunit p29
InterPro	IPR023534	Rof/RNase P-like
PFam	PF01868	Ribonuclease P/MRP, subunit p29
Superfamily	SSF101744	Rof/RNase P-like