PF3D7_0628000 (PTPS)

Genome location: Pf3D7_06_v3:1,121,527..1,122,523(+)

Genome classification: Core

Function and Localization

Product Description: 6-pyruvoyltetrahydropterin synthase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 4.2.3.12 (6-pyruvoyltetrahydropterin synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003874	6-pyruvoyltetrahydropterin synthase activity
Process	GO:0006729	tetrahydrobiopterin biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	possibly expressed	0.08	0.95
Schizont	not expressed	0.01	1.00
Gametocyte	not expressed	0.02	0.99

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0628000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.139 | MFS: -2.917 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1126700)

Relative Growth Rate: 0.92 ± 0.16
Confidence: 5.00

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1126700)

Modification: Disrupted | RMgm-3074

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KTB6

"Best" AlphaFill ligand hit: PE0 (pterine, Local RMSD=0.14) with 3M0N (Global RMSD=0.65)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.2.3.12	6-pyruvoyltetrahydropterin synthase	NEMEDTAPCMBmonoiodoacetate4-Vinylpyridineammonium sulfateN-(7-Dimethyl-amino-4-methyl coumarinyl) maleimide

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101657

Most Similar Human Ortholog: E9PNN3

TM-align score: 0.75 | RMSD: 2.25

Seq Identity: 0.20 | Length: 75 / 173

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000150787	6-pyruvoyltetrahydropterin synthase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	4	2	11
disruptive	0	4	1	10
missense	0	4	1	10

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	11	6	12
disruptive	2	11	13	34
missense	0	8	9	21

PlasmoDB Total SNPs: 55

Non-coding: 47 | Synonymous: 6 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 173 | Molecular Weight (kDa): 20.061

UniProt ID(s): C6KTB6

PDB ID(s): 1Y13

Isoelectric Point: 6.4

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007115	6-pyruvoyl tetrahydropterin synthase/QueD family
PFam	PF01242	6-pyruvoyl tetrahydropterin synthase/QueD family
Superfamily	SSF55620	N/A