Genome location: Pf3D7_06_v3:1,122,322..1,154,481(-)
Genome classification: Core
Product Description: HECT domain-containing protein 1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0061630 | ubiquitin protein ligase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.58 | 0.75 |
| Trophozoite | expressed | 0.70 | 0.69 |
| Schizont | expressed | 4.02 | 0.08 |
| Gametocyte | expressed | 1.64 | 0.35 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0628100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.669 | #Insertions: 3
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1126800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1126800)
Modification: Disrupted | RMgm-3075
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 53 | 276 | 209 | 533 |
| disruptive | 191 | 818 | 475 | 1266 |
| missense | 124 | 678 | 434 | 1137 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 91 | 630 | 308 | 624 |
| disruptive | 368 | 1584 | 814 | 1837 |
| missense | 224 | 1326 | 728 | 1530 |
PlasmoDB Total SNPs: 755
Non-coding: 88 | Synonymous: 425 | Nonsynonymous: 240 | Stop Codon: 2
Protein Length: 10287 | Molecular Weight (kDa): 1206.017
UniProt ID(s): C6KTB7
PDB ID(s): None
Isoelectric Point: 8.03
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000569 | HECT domain |
| InterPro | IPR002110 | Ankyrin repeat |
| InterPro | IPR020683 | Ankyrin repeat-containing domain |
| InterPro | IPR035983 | HECT, E3 ligase catalytic domain |
| InterPro | IPR036770 | Ankyrin repeat-containing domain superfamily |
| PFam | PF12796 | Ankyrin repeat-containing domain |
| Superfamily | SSF48403 | Ankyrin repeat-containing domain superfamily |
| Superfamily | SSF56204 | HECT, E3 ligase catalytic domain |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 31797898 | Overexpression of the HECT ubiquitin ligase PfUT prolongs the intraerythrocyticcycle and reduces invasion efficiency of Plasmodium falciparum. | Jankowska-Dollken M, Sanchez CP, Cyrklaff M, Lanzer M | 10.1038/s41598-019-54854-z |
| 32669539 | Co-option of Plasmodium falciparum PP1 for egress from host erythrocytes. | Paul AS, Miliu A, ..., Duraisingh MT | 10.1038/s41467-020-17306-1 |