PF3D7_0628300 (CEPT)

Genome location: Pf3D7_06_v3:1,169,270..1,174,371(-)

Genome classification: Core

Function and Localization

Product Description: choline/ethanolaminephosphotransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 8

EC Numbers: 2.7.8.1 (Ethanolaminephosphotransferase);2.7.8.2 (Diacylglycerol cholinephosphotransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005783	endoplasmic reticulum
Function	GO:0003723	RNA binding
Function	GO:0005515	protein binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	nd	nd
Trophozoite	no data	nd	nd
Schizont	no data	nd	nd
Gametocyte	no data	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0628300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -2.917 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1127000)

Relative Growth Rate: 0.35 ± 0.28
Confidence: 3.93

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1127000)

Modification: Tagged | RMgm-340

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KTB9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.8.1	ethanolaminephosphotransferase	BRENDA SOAP query unsuccessful
2.7.8.2	diacylglycerol cholinephosphotransferase	ATPCMPSDSCDPNaFEDTAcAMPCHAPS5'-AMP5'-UMP5'-GMPR59949...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9Y6K0	Choline/ethanolaminephosphotransferase 1		OrthoDB	CHEMBL260011CHEMBL261273CHEMBL260010CHEMBL260591...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100740

Most Similar Human Ortholog: Q9Y6K0

TM-align score: 0.86 | RMSD: 2.34

Seq Identity: 0.23 | Length: 363 / 391

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000134255	choline/ethanolamine phosphotransferase 1

Protein Information

Protein Length: 391 | Molecular Weight (kDa): 45.082

UniProt ID(s): C6KTB9

PDB ID(s): None

Isoelectric Point: 9.72

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000462	CDP-alcohol phosphatidyltransferase
InterPro	IPR014472	Choline/ethanolamine phosphotransferase
PFam	PF01066	CDP-alcohol phosphatidyltransferase
Superfamily	N/A	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	11	11	23
disruptive	1	5	2	20
missense	1	5	2	16

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	41	13	41
disruptive	2	32	31	84
missense	2	19	27	64

PlasmoDB Total SNPs: 323

Non-coding: 300 | Synonymous: 21 | Nonsynonymous: 2 | Stop Codon: 0