Genome location: Pf3D7_06_v3:1,184,868..1,189,014(-)
Genome classification: Core
Product Description: glutamyl-tRNA(Gln) amidotransferase subunit B
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 6.3.5.7 (Glutaminyl-tRNA synthase (glutamine-hydrolyzing))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0030956 | glutamyl-tRNA(Gln) amidotransferase complex |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0050567 | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity |
| Process | GO:0070681 | glutaminyl-tRNAGln biosynthesis via transamidation |
| Process | GO:0006424 | glutamyl-tRNA aminoacylation |
| Process | GO:0032543 | mitochondrial translation |
| Process | GO:0006412 | translation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.05 | 0.97 |
| Trophozoite | no data | 0.47 | 0.72 |
| Schizont | no data | 0.08 | 0.95 |
| Gametocyte | no data | 0.21 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0628800
Resistome Missense Mutations: E266V, V271L
Resistome Compounds with Missense Mutations: DDD107498
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.2 | MFS: -2.724 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1127500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KTC3
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=1.32) with 3H0R (Global RMSD=6.33)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.5.7 | glutaminyl-tRNA synthase (glutamine-hydrolysing) | ADPpuromycinATP-gammaSchloramphenicolgamma-Glu boronic acid6-diazo-5-oxonorleucineL-methionine-S-sulfoximine2'-O-(trinitrophenyl)adenosine 5'-triphosphate3'-O-(trinitrophenyl)adenosine 5'-triphosphateadenosine 5'-[beta,gamma-methylene]triphosphate3'-deoxy-3'-(L-glutaminylamino)-N,N-dimethyladenosine3'-(L-alpha-aspartylamino)-3'-deoxy-N,N-dimethyladenosine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101213
Most Similar Human Ortholog: D6RDU9
TM-align score: 0.70 | RMSD: 3.03
Seq Identity: 0.30 | Length: 280 / 882
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000059691 | glutamyl-tRNA amidotransferase subunit B |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 14 | 13 | 31 |
| disruptive | 10 | 56 | 21 | 104 |
| missense | 6 | 44 | 19 | 85 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 31 | 36 | 33 |
| disruptive | 26 | 122 | 69 | 159 |
| missense | 14 | 88 | 52 | 119 |
PlasmoDB Total SNPs: 198
Non-coding: 90 | Synonymous: 48 | Nonsynonymous: 60 | Stop Codon: 0
Protein Length: 882 | Molecular Weight (kDa): 104.977
UniProt ID(s): C6KTC3
PDB ID(s): None
Isoelectric Point: 6.42
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003789 | Aspartyl/glutamyl-tRNA amidotransferase subunit B-like |
| InterPro | IPR006075 | Aspartyl/Glutamyl-tRNA(Gln) amidotransferase, subunit B/E, catalytic |
| InterPro | IPR014746 | Glutamine synthetase/guanido kinase, catalytic domain |
| InterPro | IPR017959 | Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, subunit B /E |
| InterPro | IPR018027 | Asn/Gln amidotransferase |
| PFam | PF02637 | Asn/Gln amidotransferase |
| PFam | PF02934 | Aspartyl/Glutamyl-tRNA(Gln) amidotransferase, subunit B/E, catalytic |
| Superfamily | SSF55931 | Glutamine synthetase/guanido kinase, catalytic domain |
| Superfamily | SSF89095 | Aspartyl/glutamyl-tRNA amidotransferase subunit B-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 26318454 | Plasmodium Apicoplast Gln-tRNAGln Biosynthesis Utilizes a Unique GatABAmidotransferase Essential for Erythrocytic Stage Parasites. | Mailu BM, Li L, ..., Gardner MJ | 10.1074/jbc.M115.655100 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 35369065 | Analysis of Lactobacillus rhamnosus GG in Mulberry Galacto-Oligosaccharide Mediumby Comparative Transcriptomics and Metabolomics | Li E, Zhu Q, ..., Zou Y | 10.3389/fnut.2022.853271 |
| 33072044 | Forward Genetics Reveals a gatC-gatA Fusion Polypeptide Causes Mistranslation and Rifampicin Tolerance in Mycobacterium smegmatis | Cai RJ, Su HW, Li YY, Javid B | 10.3389/fmicb.2020.577756 |