Product Description: phospholipase, putative
SignalP Peptide: MSSILLFFVVFQYLLISFS
# Transmembrane Domains: 0
EC Numbers: 2.3.1.43 (Phosphatidylcholine--sterol O-acyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0016787 | hydrolase activity |
| Function | GO:0016298 | lipase activity |
| Function | GO:0004607 | phosphatidylcholine-sterol O-acyltransferase activity |
| Function | GO:0004620 | phospholipase activity |
| Function | GO:0004806 | triglyceride lipase activity |
| Process | GO:0044409 | entry into host |
| Process | GO:0035891 | exit from host cell |
| Process | GO:0006629 | lipid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.08 | 0.96 |
| Trophozoite | no data | 0.05 | 0.97 |
| Schizont | no data | 0.41 | 0.82 |
| Gametocyte | no data | 0.09 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0629300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.425 | MFS: -2.636 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1128100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1128100)
Modification: Disrupted | RMgm-3082
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KTC8
"Best" AlphaFill ligand hit: MAY (mafp, Local RMSD=0.46) with 4X97 (Global RMSD=3.63)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.43 | phosphatidylcholine-sterol O-acyltransferase | DTNBcysteinecarnitinetunicamycinHaptoglobinTriton X-100lysolecithinsphingomyelinantibody 25B7Serum albumindithiothreitolchlorpromazine... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P04180 | Phosphatidylcholine-sterol acyltransferase | Homo sapiens | OrthoDB, OrthoMCL BLAST | Difluoroketone phospholipid analogue, 2Difluoroketone phospholipid analogue, 3Difluoroketone phospholipid analogue, 4 |
| A0A068N674 | Pgap1 family protein | OrthoMCL BLAST | US10138240, Example 6US10138240, Example 6 |
Ortholog Group (OrthoMCL): OG6_101376
Most Similar Human Ortholog: J3QKT0
TM-align score: 0.91 | RMSD: 1.59
Seq Identity: 0.35 | Length: 136 / 863
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000103066 | phospholipase A2 group XV |
| ENSG00000213398 | lecithin-cholesterol acyltransferase |
Protein Length: 863 | Molecular Weight (kDa): 99.231
UniProt ID(s): C6KTC8
PDB ID(s): None
Isoelectric Point: 4.64
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003386 | Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase |
| InterPro | IPR029058 | Alpha/Beta hydrolase fold |
| PFam | PF02450 | Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase |
| Superfamily | SSF53474 | Alpha/Beta hydrolase fold |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 14 | 12 | 26 |
| disruptive | 17 | 25 | 18 | 45 |
| missense | 9 | 20 | 14 | 37 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 36 | 22 | 35 |
| disruptive | 31 | 56 | 35 | 122 |
| missense | 16 | 40 | 28 | 86 |
PlasmoDB Total SNPs: 248
Non-coding: 51 | Synonymous: 90 | Nonsynonymous: 103 | Stop Codon: 4
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15590623 | A surface phospholipase is involved in the migration of plasmodium sporozoitesthrough cells. | Bhanot P, Schauer K, Coppens I, Nussenzweig V | 10.1074/jbc.M411465200 |
| 25786000 | A Plasmodium phospholipase is involved in disruption of the liver stageparasitophorous vacuole membrane. | Burda PC, Roelli MA, ..., Heussler VT | 10.1371/journal.ppat.1004760 |