About Gene List

PF3D7_0629500 (AAT1)

Genome location: Pf3D7_06_v3:1,213,102..1,217,313(-)

Genome classification: Core

Function and Localization

Product Description: amino acid transporter AAT1

SignalP Peptide: N/A

# Transmembrane Domains: 9

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020020 food vacuole
Component GO:0005886 plasma membrane
Function GO:0015171 amino acid transmembrane transporter activity
Function GO:0042910 xenobiotic transmembrane transporter activity
Process GO:0003333 amino acid transmembrane transport
Process GO:0006865 amino acid transport
Process GO:1902349 response to chloroquine
Process GO:0009410 response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.77 0.64
Trophozoite expressed 0.70 0.62
Schizont expressed 0.17 0.90
Gametocyte expressed 0.27 0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0629500

Resistome Missense Mutations: V185L, F230L, K238N, F377L, P380S

Resistome Compounds with Missense Mutations: MMV006901, MMV011895, MMV668399

Resistome # Samples with Disruptive Mutations: 9 (8 missense, 8 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.143 | MFS: -2.882 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1128300)

Modification: Disrupted | RMgm-5357

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KTD0

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_124658

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 2 15 8 28
disruptive 8 24 11 54
missense 8 23 11 43

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 4 38 29 37
disruptive 21 69 54 142
missense 13 56 50 82

PlasmoDB Total SNPs: 162

Non-coding: 141 | Synonymous: 14 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 606 | Molecular Weight (kDa): 68.858

UniProt ID(s): C6KTD0

PDB ID(s): None

Isoelectric Point: 10.15

Protein Domain Annotations:

Source Family ID Description
InterPro IPR013057 Amino acid transporter, transmembrane domain
PFam PF01490 Amino acid transporter, transmembrane domain
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
29326268 Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics. Cowell AN, Istvan ES, ..., Winzeler EA 10.1126/science.aan4472
29410428 Heterologous Expression of a Novel Drug Transporter from the Malaria ParasiteAlters Resistance to Quinoline Antimalarials. Tindall SM, Vallieres C, ..., Avery SV 10.1038/s41598-018-20816-0
35121625 Environmental control of Pub1 (NEDD4 family E3 ligase) in Schizosaccharomycespombe is regulated by TORC2 and Gsk3 Wang T, Woodman P, Humphrey SJ, Petersen J 10.26508/lsa.202101082
37169919 Chloroquine resistance evolution in Plasmodium falciparum is mediated by theputative amino acid transporter AAT1 Amambua-Ngwa A, Button-Simons KA, ..., Anderson TJC 10.1038/s41564-023-01377-z
35368081 In silico target-based strain engineering of Saccharomyces cerevisiae for terpeneprecursor improvement Paramasivan K, Abdulla A, Gupta N, Mutturi S 10.1093/intbio/zyac003
36542676 Resolving drug selection and migration in an inbred South American Plasmodiumfalciparum population with identity-by-descent analysis Carrasquilla M, Early AM, ..., Corredor V 10.1371/journal.ppat.1010993