PF3D7_0630300

Genome location: Pf3D7_06_v3:1,260,163..1,270,722(-)

Genome classification: Core

Function and Localization

Product Description: DNA polymerase epsilon catalytic subunit A, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.7 (DNA-directed DNA polymerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0008622	epsilon DNA polymerase complex
Function	GO:0003677	DNA binding
Function	GO:0003887	DNA-directed DNA polymerase activity
Function	GO:0008310	single-stranded DNA 3'-5' exodeoxyribonuclease activity
Process	GO:0045004	DNA replication proofreading
Process	GO:0006261	DNA-dependent DNA replication
Process	GO:0006287	base-excision repair, gap-filling
Process	GO:0006272	leading strand elongation
Process	GO:0000278	mitotic cell cycle
Process	GO:0006297	nucleotide-excision repair, DNA gap filling

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.12	0.94
Trophozoite	possibly expressed	0.96	0.52
Schizont	expressed	0.37	0.79
Gametocyte	expressed	0.92	0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0630300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.122 | MFS: -3.04 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1129100)

Relative Growth Rate: 0.34 ± 0.44
Confidence: 3.02

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.7	DNA-directed DNA polymerase	KClEDTANH4+dATPNH4ClddTTPMK886UracilalliinsucroseethanolAllicin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101586

Most Similar Human Ortholog: Not available

TM-align score: 0.00 | RMSD: 0.00

Seq Identity: 0.00 | Length: / 2907

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000177084	DNA polymerase epsilon, catalytic subunit

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	41	108	49	175
disruptive	55	165	90	250
missense	29	135	71	196

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	59	236	83	150
disruptive	111	379	195	409
missense	55	264	156	273

PlasmoDB Total SNPs: 312

Non-coding: 153 | Synonymous: 122 | Nonsynonymous: 37 | Stop Codon: 0

Protein Information

Protein Length: 2907 | Molecular Weight (kDa): 344.607

UniProt ID(s): C6KTD8

PDB ID(s): None

Isoelectric Point: 8.55

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006133	DNA-directed DNA polymerase, family B, exonuclease domain
InterPro	IPR006172	DNA-directed DNA polymerase, family B
InterPro	IPR012337	Ribonuclease H-like superfamily
InterPro	IPR013697	DNA polymerase epsilon, catalytic subunit A, C-terminal
InterPro	IPR029703	DNA polymerase epsilon catalytic subunit
InterPro	IPR043502	DNA/RNA polymerase superfamily
PFam	PF03104	DNA-directed DNA polymerase, family B, exonuclease domain
Superfamily	SSF53098	Ribonuclease H-like superfamily
Superfamily	SSF56672	DNA/RNA polymerase superfamily

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104