Genome location: Pf3D7_06_v3:1,290,623..1,293,448(-)
Genome classification: Core
Product Description: ATP-dependent RNA helicase HAS1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005730 | nucleolus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
| Process | GO:0032508 | DNA duplex unwinding |
| Process | GO:0016070 | RNA metabolic process |
| Process | GO:0010501 | RNA secondary structure unwinding |
| Process | GO:0000463 | maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.88 | 0.60 |
| Trophozoite | expressed | 0.75 | 0.62 |
| Schizont | expressed | 0.18 | 0.89 |
| Gametocyte | expressed | 0.44 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0630900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.188 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1129700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KTE4
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.21) with 3RRM (Global RMSD=3.21)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102072
Most Similar Human Ortholog: H7C452
TM-align score: 0.85 | RMSD: 2.60
Seq Identity: 0.57 | Length: 212 / 601
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000088205 | DEAD-box helicase 18 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 32 | 13 | 34 |
| disruptive | 17 | 45 | 26 | 58 |
| missense | 11 | 34 | 16 | 52 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 67 | 12 | 33 |
| disruptive | 34 | 95 | 46 | 94 |
| missense | 17 | 67 | 37 | 69 |
PlasmoDB Total SNPs: 117
Non-coding: 59 | Synonymous: 39 | Nonsynonymous: 19 | Stop Codon: 0
Protein Length: 601 | Molecular Weight (kDa): 69.359
PDB ID(s): None
Isoelectric Point: 8.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000629 | ATP-dependent RNA helicase DEAD-box, conserved site |
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR025313 | Domain of unknown function DUF4217 |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| PFam | PF13959 | Domain of unknown function DUF4217 |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 20153446 | A novel DEAD box helicase Has1p from Plasmodium falciparum: N-terminal isessential for activity. | Prakash K, Tuteja R | 10.1016/j.parint.2010.02.003 |