Genome location: Pf3D7_06_v3:1,353,946..1,366,430(-)
Genome classification: SubtelomericHypervariable
Product Description: erythrocyte membrane protein 1, PfEMP1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0020030 | infected host cell surface knob |
| Function | GO:0050839 | cell adhesion molecule binding |
| Process | GO:0020033 | antigenic variation |
| Process | GO:0098609 | cell-cell adhesion |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
| Process | GO:0020013 | modulation by symbiont of host erythrocyte aggregation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.01 | 0.99 |
| Schizont | possibly expressed | 0.01 | 0.99 |
| Gametocyte | possibly expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0632500
Resistome Missense Mutations: E384K, E384D, E805D, I806N, V827I, T3917A
Resistome Compounds with Missense Mutations: AN13762, AN13956
Resistome # Samples with Disruptive Mutations: 11 (7 missense, 7 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.051 | #Insertions: 6
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 4291
Non-coding: 276 | Synonymous: 979 | Nonsynonymous: 3023 | Stop Codon: 13
Protein Length: 3954 | Molecular Weight (kDa): 456.918
UniProt ID(s): A0A191VZ28, A0A191VZ43, A0A191VZ68, A0A5P0ZZX9, A0A5P1A0A7, A0A5P1A0H2, A0A5P1A0J8, A0A5P1A0N0, A0A5P1A0N8, A0A5P1A0U0, A0A5P1A0Y6, A0A5P1A108, A0A5P1A110, A0A5P1A138, A0A5P1A162, A0A5P1A185, A0A5P1A279, A0A5P1A2C3, A0A5P1A3C4, A0A5P1A3D5, A1KQT2, A3R6S9, A3R6T8, C6KTF7, Q26031, Q95W83
PDB ID(s): None
Isoelectric Point: 6.19
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004258 | Duffy-binding-like domain |
| InterPro | IPR008602 | Duffy-antigen binding |
| InterPro | IPR029210 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| InterPro | IPR029211 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| PFam | PF03011 | Duffy-binding-like domain |
| PFam | PF05424 | Duffy-antigen binding |
| PFam | PF15445 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| PFam | PF15447 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| Superfamily | SSF140924 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9230440 | P. falciparum rosetting mediated by a parasite-variant erythrocyte membraneprotein and complement-receptor 1. | Rowe JA, Moulds JM, Newbold CI, Miller LH | 10.1038/40888 |
| 9291676 | Knob proteins in falciparum malaria. | Sharma YD | https://pubmed.ncbi.nlm.nih.gov/9291676/ |
| 9786187 | A recombinant peptide based on PfEMP-1 blocks and reverses adhesion ofmalaria-infected red blood cells to CD36 under flow. | Cooke BM, Nicoll CL, Baruch DI, Coppel RL | 10.1046/j.1365-2958.1998.01040.x |
| 11316367 | Variant antigens of Plasmodium falciparum encoded by the var multigenic familyare multifunctional macromolecules. | Nogueira PA, Wunderlich G, Pereira da Silva LH | 10.1016/s0923-2508(01)01184-6 |
| 11420100 | Molecules on the surface of the Plasmodium falciparum infected erythrocyte andtheir role in malaria pathogenesis and immune evasion. | Craig A, Scherf A | https://pubmed.ncbi.nlm.nih.gov/11420100/ |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 16455655 | Strict pairing of var promoters and introns is required for var gene silencing inthe malaria parasite Plasmodium falciparum. | Frank M, Dzikowski R, ..., Deitsch K | 10.1074/jbc.M513067200 |
| 16896206 | Programmed transcription of the var gene family, but not of stevor, in Plasmodiumfalciparum gametocytes. | Sharp S, Lavstsen T, ..., Sutherland CJ | 10.1128/EC.00029-06 |
| 17257309 | Plasmodium falciparum var gene expression is developmentally controlled at thelevel of RNA polymerase II-mediated transcription initiation. | Kyes S, Christodoulou Z, ..., Newbold C | 10.1111/j.1365-2958.2007.05587.x |
| 17762879 | Mechanisms underlying mutually exclusive expression of virulence genes by malariaparasites. | Dzikowski R, Li F, ..., Deitsch KW | 10.1038/sj.embor.7401063 |
| 18433451 | Plasmodium falciparum antigenic variation. Mapping mosaic var gene sequences ontoa network of shared, highly polymorphic sequence blocks. | Bull PC, Buckee CO, ..., Marsh K | 10.1111/j.1365-2958.2008.06248.x |
| 18785843 | Antigenic variation in Plasmodium falciparum. | Scherf A, Lopez-Rubio JJ, Riviere L | 10.1146/annurev.micro.61.080706.093134 |
| 20509952 | Identification of a major rif transcript common to gametocytes and sporozoites ofPlasmodium falciparum. | Wang CW, Mwakalinga SB, ..., Lavstsen T | 10.1186/1475-2875-9-147 |
| 20540611 | Differential var gene expression in children with malaria and antidromic effectson host gene expression. | Kalmbach Y, Rottmann M, ..., Kun JF | 10.1086/653586 |
| 21317310 | Plasmodium falciparum var gene silencing is determined by cis DNA elements thatform stable and heritable interactions. | Swamy L, Amulic B, Deitsch KW | 10.1128/EC.00329-10 |
| 22100161 | A critical role of perinuclear filamentous actin in spatial repositioning andmutually exclusive expression of virulence genes in malaria parasites. | Zhang Q, Huang Y, ..., Scherf A | 10.1016/j.chom.2011.09.013 |
| 22221747 | Analysis of var genes cloned from a Plasmodium falciparum isolate in China. | Jiang N, Meng L, ..., Chen QJ | 10.1016/S1995-7645(12)60001-7 |
| 22461905 | Erasing the epigenetic memory and beginning to switch--the onset of antigenicswitching of var genes in Plasmodium falciparum. | Fastman Y, Noble R, Recker M, Dzikowski R | 10.1371/journal.pone.0034168 |
| 23297413 | Molecular architecture of a complex between an adhesion protein from the malariaparasite and intracellular adhesion molecule 1. | Brown A, Turner L, ..., Higgins MK | 10.1074/jbc.M112.416347 |
| 23825944 | DC8 and DC13 var genes associated with severe malaria bind avidly to diverseendothelial cells. | Avril M, Brazier AJ, ..., Smith JD | 10.1371/journal.ppat.1003430 |
| 23980881 | Genome-wide profiling of chromosome interactions in Plasmodium falciparumcharacterizes nuclear architecture and reconfigurations associated with antigenicvariation. | Lemieux JE, Kyes SA, ..., Newbold CI | 10.1111/mmi.12381 |
| 25691743 | Antisense long noncoding RNAs regulate var gene activation in the malariaparasite Plasmodium falciparum. | Amit-Avraham I, Pozner G, ..., Dzikowski R | 10.1073/pnas.1420855112 |
| 26456841 | Ape parasite origins of human malaria virulence genes. | Larremore DB, Sundararaman SA, ..., Buckee CO | 10.1038/ncomms9368 |
| 27538502 | Punctuated chromatin states regulate Plasmodium falciparum antigenic variation atthe intron and 2 kb upstream regions. | Wang C, Adapa SR, ..., Jiang RH | 10.1186/s12864-016-3005-7 |
| 27860263 | Activation and clustering of a Plasmodium falciparum var gene are affected bysubtelomeric sequences. | Duffy MF, Tang J, ..., Brown GV | 10.1111/febs.13967 |