PF3D7_0702200 (LPL20)

Genome location: Pf3D7_07_v3:91,731..95,602(-)

Genome classification: Core

Function and Localization

Product Description: lysophospholipase LPL20

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.1.5 (Lysophospholipase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0071944	cell periphery
Component	GO:0005829	cytosol
Component	GO:0016020	membrane
Component	GO:0031982	vesicle
Function	GO:0016787	hydrolase activity
Function	GO:0016298	lipase activity
Function	GO:0004622	lysophospholipase activity
Process	GO:0046475	glycerophospholipid catabolic process
Process	GO:0006644	phospholipid metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.50	0.77
Trophozoite	expressed	0.07	0.96
Schizont	expressed	0.05	0.97
Gametocyte	possibly expressed	0.03	0.98

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0702200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.889 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1220300)

Relative Growth Rate: 1.09 ± 0.18
Confidence: 4.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IC45

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.1.5	lysophospholipase	NEMSDSEDTAPCMBEGTAPMSFDMSOCaCl2FeCl3xyleneethanolsulfite...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100231

Most Similar Human Ortholog: A0A3B3ITT3

TM-align score: 0.86 | RMSD: 1.95

Seq Identity: 0.21 | Length: 242 / 424

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000074416	monoglyceride lipase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	1	0	0
disruptive	0	0	0	0
missense	0	0	0	0

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	0	0	0
disruptive	0	0	0	0
missense	0	0	0	0

PlasmoDB Total SNPs: 186

Non-coding: 154 | Synonymous: 26 | Nonsynonymous: 4 | Stop Codon: 2

Protein Information

Protein Length: 424 | Molecular Weight (kDa): 49.243

UniProt ID(s): Q8IC45

PDB ID(s): None

Isoelectric Point: 5.03

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006494	Plasmodium subtelomeric PST-A
InterPro	IPR022742	Serine aminopeptidase, S33
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF12146	Serine aminopeptidase, S33
Superfamily	SSF53474	Alpha/Beta hydrolase fold