PF3D7_0703500

Genome location: Pf3D7_07_v3:133,470..143,247(+)

Genome classification: Core

Function and Localization

Product Description: erythrocyte membrane-associated antigen

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016020	membrane
Component	GO:0005634	nucleus
Function	GO:0003723	RNA binding
Function	GO:0003729	mRNA binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.90	0.28
Trophozoite	expressed	1.92	0.20
Schizont	expressed	1.04	0.49
Gametocyte	expressed	1.43	0.34

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0703500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.122 | MFS: -2.946 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IC35

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.03) with 8FTM (Global RMSD=2.42)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_136626

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	47	175	65	168
disruptive	93	302	124	349
missense	41	220	101	258

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	73	322	92	149
disruptive	174	578	239	577
missense	76	434	167	327

PlasmoDB Total SNPs: 411

Non-coding: 159 | Synonymous: 158 | Nonsynonymous: 94 | Stop Codon: 0

Protein Information

Protein Length: 2299 | Molecular Weight (kDa): 264.916

UniProt ID(s): Q8IC35, Q9GVB0

PDB ID(s): None

Isoelectric Point: 8.85

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR041677	DNA2/NAM7 helicase, helicase domain
InterPro	IPR041679	DNA2/NAM7 helicase-like, C-terminal
PFam	PF13086	DNA2/NAM7 helicase, helicase domain
PFam	PF13087	DNA2/NAM7 helicase-like, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
27381095	The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum.	Bunnik EM, Batugedara G, ..., Le Roch KG	10.1186/s13059-016-1014-0
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596