PF3D7_0704500

Genome location: Pf3D7_07_v3:207,891..213,784(-)

Genome classification: Core

Function and Localization

Product Description: serine/threonine protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:1903561	extracellular vesicle

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.10	0.95
Trophozoite	expressed	0.28	0.86
Schizont	expressed	1.42	0.46
Gametocyte	expressed	0.45	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0704500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.787 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0802200)

Modification: Disrupted | RMgm-2292

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4K5

"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=0.22) with 5A6N (Global RMSD=2.86)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533317

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	19	67	46	99
disruptive	68	271	132	326
missense	53	224	114	285

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	27	163	71	101
disruptive	131	559	245	428
missense	86	459	196	284

PlasmoDB Total SNPs: 286

Non-coding: 103 | Synonymous: 91 | Nonsynonymous: 92 | Stop Codon: 0

Protein Information

Protein Length: 1603 | Molecular Weight (kDa): 192.854

UniProt ID(s): C0H4K5

PDB ID(s): None

Isoelectric Point: 9.73

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
PFam	PF00069	Protein kinase domain
Superfamily	SSF56112	Protein kinase-like domain superfamily