Product Description: HECT-type E3 ubiquitin ligase UT
SignalP Peptide: N/A
# Transmembrane Domains: 4
EC Numbers: 2.3.2.26 (HECT-type E3 ubiquitin transferase);6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0004842 | ubiquitin-protein transferase activity |
| Process | GO:0042493 | response to drug |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.64 | 0.37 |
| Trophozoite | expressed | 2.37 | 0.12 |
| Schizont | expressed | 2.40 | 0.18 |
| Gametocyte | expressed | 2.55 | 0.10 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0704600
Resistome Missense Mutations: N862I, S1260R
Resistome Compounds with Missense Mutations: AN13762, WLW
Resistome # Samples with Disruptive Mutations: 3 (2 missense, 1 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.305 | #Insertions: 4
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0802300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.2.26 | HECT-type E3 ubiquitin transferase | CalmodulinclomipraminechlorpromazinechlorprothixenenorclomipramineN-acetylphenylalanylamide4-[[(3-chloro-1,4-dioxo-1,4-dihydronaphthalen-2-yl)amino]methyl]-N-(pyridin-4-yl)benzamide |
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102181
Most Similar Human Ortholog: Not available
TM-align score: 0.00 | RMSD: 0.00
Seq Identity: 0.00 | Length: / 3893
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000153827 | thyroid hormone receptor interactor 12 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 68 | 200 | 133 | 254 |
| disruptive | 198 | 598 | 352 | 825 |
| missense | 121 | 479 | 310 | 708 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 93 | 421 | 177 | 268 |
| disruptive | 359 | 1220 | 628 | 1176 |
| missense | 199 | 993 | 521 | 845 |
PlasmoDB Total SNPs: 829
Non-coding: 428 | Synonymous: 202 | Nonsynonymous: 199 | Stop Codon: 0
Protein Length: 3893 | Molecular Weight (kDa): 460.42
UniProt ID(s): C0H4K6
PDB ID(s): None
Isoelectric Point: 6.53
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000569 | HECT domain |
| InterPro | IPR016024 | Armadillo-type fold |
| InterPro | IPR035983 | HECT, E3 ligase catalytic domain |
| PFam | PF00632 | HECT domain |
| Superfamily | SSF48371 | Armadillo-type fold |
| Superfamily | SSF56204 | HECT, E3 ligase catalytic domain |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 24830312 | A HECT ubiquitin-protein ligase as a novel candidate gene for altered quinine andquinidine responses in Plasmodium falciparum. | Sanchez CP, Liu CH, ..., Lanzer M | 10.1371/journal.pgen.1004382 |
| 31797898 | Overexpression of the HECT ubiquitin ligase PfUT prolongs the intraerythrocyticcycle and reduces invasion efficiency of Plasmodium falciparum. | Jankowska-Dollken M, Sanchez CP, Cyrklaff M, Lanzer M | 10.1038/s41598-019-54854-z |