PF3D7_0704800 (PPM12)

Genome location: Pf3D7_07_v3:237,810..242,106(-)

Genome classification: Core

Function and Localization

Product Description: protein phosphatase PPM12, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.18	0.91
Trophozoite	expressed	0.39	0.79
Schizont	expressed	0.97	0.49
Gametocyte	expressed	0.38	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0704800

Resistome Missense Mutations: I323V

Resistome Compounds with Missense Mutations: Artemisinin

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.628 | #Insertions: 4

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0802500)

Modification: Disrupted | RMgm-2294

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IC22

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.3.16	protein-serine/threonine phosphatase	ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_137272

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	53	47	72
disruptive	66	204	101	221
missense	48	158	87	196

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	127	63	73
disruptive	124	397	174	352
missense	85	314	137	238

PlasmoDB Total SNPs: 164

Non-coding: 29 | Synonymous: 57 | Nonsynonymous: 78 | Stop Codon: 0

Protein Information

Protein Length: 1297 | Molecular Weight (kDa): 152.656

UniProt ID(s): Q8IC22

PDB ID(s): None

Isoelectric Point: 6.88

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001646	Pentapeptide repeat
InterPro	IPR036457	PPM-type phosphatase domain superfamily
InterPro	IPR039123	Protein phosphatase 2C
PFam	PF13599	Pentapeptide repeat
Superfamily	SSF141571	PPM-type phosphatase domain superfamily
Superfamily	SSF81606