Product Description: peptide chain release factor 2
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 3.1.1.29 (Aminoacyl-tRNA hydrolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0043022 | ribosome binding |
| Function | GO:0003747 | translation release factor activity |
| Process | GO:0006415 | translational termination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | not expressed | 0.31 | 0.80 |
| Schizont | not expressed | 0.10 | 0.93 |
| Gametocyte | possibly expressed | 0.46 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0704900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.126 | MFS: -2.841 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0802600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0802600)
Modification: Disrupted | RMgm-2295
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IC21
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.1.29 | aminoacyl-tRNA hydrolase | EDTAtRNAtRNALystRNAfMettRNA-lysine5-azacytidine70 S ribosomeUncharged tRNAdeacetylated tRNAdiethyldicarbonatenon-esterified tRNALystRNA-formyl-methionine... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 19 | 24 | 23 |
| disruptive | 4 | 16 | 16 | 39 |
| missense | 2 | 14 | 15 | 38 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 54 | 10 | 20 |
| disruptive | 7 | 54 | 28 | 92 |
| missense | 3 | 44 | 22 | 66 |
PlasmoDB Total SNPs: 15
Non-coding: 0 | Synonymous: 8 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 372 | Molecular Weight (kDa): 43.02
UniProt ID(s): Q8IC21
PDB ID(s): None
Isoelectric Point: 10.14
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000352 | Peptide chain release factor class I |
| InterPro | IPR005139 | Peptide chain release factor |
| PFam | PF00472 | Peptide chain release factor class I |
| PFam | PF03462 | Peptide chain release factor |
| Superfamily | SSF75620 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 26946524 | Polypeptide release factors and stop codon recognition in the apicoplast andmitochondrion of Plasmodium falciparum. | Vaishya S, Kumar V, ..., Habib S | 10.1111/mmi.13369 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |