PF3D7_0705000

Genome location: Pf3D7_07_v3:245,497..250,602(-)

Genome classification: Core

Function and Localization

Product Description: mRNA cap guanine-N7 methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.56 (mRNA (guanine-N(7)-)-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005845	mRNA cap binding complex
Component	GO:0005634	nucleus
Function	GO:0004482	mRNA (guanine-N7-)-methyltransferase activity
Process	GO:0006370	7-methylguanosine mRNA capping

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.86	0.62
Trophozoite	expressed	1.18	0.41
Schizont	expressed	0.47	0.73
Gametocyte	expressed	0.77	0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0705000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.126 | MFS: -2.834 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0802700)

Relative Growth Rate: 0.15 ± 0.61
Confidence: 2.36

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IC20

"Best" AlphaFill ligand hit: OPE (phosphoric acid mono-(2-amino-ethyl) ester, Local RMSD=0.09) with 3UJA (Global RMSD=2.62)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.56	mRNA (guanine-N7)-methyltransferase	EDTAGpppGThesitribavirinDigitoninsinefunginTriton X-100deoxycholatecycloheximideS-adenosyl-L-cysteineaurintricarboxylic acidS-adenosyl-homocysteine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102244

Most Similar Human Ortholog: K7EP06

TM-align score: 0.78 | RMSD: 2.35

Seq Identity: 0.21 | Length: 255 / 1213

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000101654	RNA guanine-7 methyltransferase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	40	42	69
disruptive	17	95	62	147
missense	17	88	60	143

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	113	51	52
disruptive	35	220	118	225
missense	28	197	109	171

PlasmoDB Total SNPs: 184

Non-coding: 97 | Synonymous: 63 | Nonsynonymous: 23 | Stop Codon: 1

Protein Information

Protein Length: 1213 | Molecular Weight (kDa): 145.172

UniProt ID(s): Q8IC20

PDB ID(s): None

Isoelectric Point: 6.88

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004971	mRNA (guanine-N(7))-methyltransferase domain
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
InterPro	IPR039753	mRNA cap guanine-N7 methyltransferase
PFam	PF03291	mRNA (guanine-N(7))-methyltransferase domain
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase