Product Description: DNA replication licensing factor MCM7
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0042555 | MCM complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0008094 | ATP-dependent activity, acting on DNA |
| Function | GO:0003688 | DNA replication origin binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Process | GO:0006270 | DNA replication initiation |
| Process | GO:0006271 | DNA strand elongation involved in DNA replication |
| Process | GO:0006268 | DNA unwinding involved in DNA replication |
| Process | GO:0000727 | double-strand break repair via break-induced replication |
| Process | GO:0006267 | pre-replicative complex assembly involved in nuclear cell cycle DNA replication |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.75 | 0.59 |
| Schizont | expressed | 0.73 | 0.59 |
| Gametocyte | expressed | 0.72 | 0.61 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0705400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.222 | MFS: -2.769 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0803100)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_0805800)
Modification: Tagged | RMgm-4983
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IC16
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.21) with 4R7Y (Global RMSD=8.91)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102101
Most Similar Human Ortholog: C9J8M6
TM-align score: 0.85 | RMSD: 2.41
Seq Identity: 0.36 | Length: 171 / 821
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000166508 | minichromosome maintenance complex component 7 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 35 | 22 | 40 |
| disruptive | 6 | 17 | 17 | 45 |
| missense | 6 | 16 | 16 | 38 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 77 | 23 | 42 |
| disruptive | 6 | 54 | 38 | 112 |
| missense | 6 | 44 | 31 | 81 |
PlasmoDB Total SNPs: 81
Non-coding: 65 | Synonymous: 9 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 821 | Molecular Weight (kDa): 94.237
UniProt ID(s): Q8IC16
PDB ID(s): None
Isoelectric Point: 6.27
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001208 | MCM domain |
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR008050 | DNA replication licensing factor Mcm7 |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR018525 | Mini-chromosome maintenance, conserved site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR027925 | MCM N-terminal domain |
| InterPro | IPR031327 | Mini-chromosome maintenance protein |
| InterPro | IPR033762 | MCM OB domain |
| InterPro | IPR041562 | MCM, AAA-lid domain |
| PFam | PF00493 | MCM domain |
| PFam | PF14551 | MCM N-terminal domain |
| PFam | PF17207 | MCM OB domain |
| PFam | PF17855 | MCM, AAA-lid domain |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16257456 | Pre-replication complex organization in the atypical DNA replication cycle ofPlasmodium falciparum: characterization of the mini-chromosome maintenance (MCM)complex formation. | Patterson S, Robert C, ..., Chakrabarti D | 10.1016/j.molbiopara.2005.09.006 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |