PF3D7_0705600

Genome location: Pf3D7_07_v3:280,570..284,888(-)

Genome classification: Core

Function and Localization

Product Description: RNA helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Component	GO:1990904	ribonucleoprotein complex
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.20	0.90
Trophozoite	no data	0.44	0.75
Schizont	no data	0.06	0.96
Gametocyte	no data	0.16	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0705600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.181 | MFS: -2.822 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0803300)

Relative Growth Rate: 0.07 ± 0.11
Confidence: 5.82

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4K7

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.13) with 5JCH (Global RMSD=4.28)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_133057

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	12	59	37	64
disruptive	26	139	98	196
missense	25	132	88	174

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	127	44	74
disruptive	64	335	147	299
missense	49	290	129	215

PlasmoDB Total SNPs: 137

Non-coding: 51 | Synonymous: 49 | Nonsynonymous: 37 | Stop Codon: 0

Protein Information

Protein Length: 1132 | Molecular Weight (kDa): 136.149

UniProt ID(s): C0H4K7

PDB ID(s): None

Isoelectric Point: 10.09

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase