PF3D7_0706700 (MSH2-2)

Genome location: Pf3D7_07_v3:322,907..325,528(+)

Genome classification: Core

Function and Localization

Product Description: DNA mismatch repair protein MSH2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0032301	MutSalpha complex
Component	GO:0032302	MutSbeta complex
Component	GO:0032300	mismatch repair complex
Component	GO:0005634	nucleus
Function	GO:0008094	ATP-dependent activity, acting on DNA
Function	GO:0000406	double-strand/single-strand DNA junction binding
Process	GO:0006310	DNA recombination
Process	GO:0043570	maintenance of DNA repeat elements
Process	GO:0006298	mismatch repair
Process	GO:0045910	negative regulation of DNA recombination
Process	GO:0006301	postreplication repair

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.09	0.95
Trophozoite	not expressed	0.07	0.96
Schizont	possibly expressed	0.07	0.95
Gametocyte	expressed	0.37	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0706700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.444 | #Insertions: 4

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1017100)

Relative Growth Rate: 0.43 ± 0.21
Confidence: 4.53

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0804300)

Modification: Disrupted | RMgm-300

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4L8

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.19) with 2WTU (Global RMSD=1.93)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102031

Most Similar Human Ortholog: P43246

TM-align score: 0.79 | RMSD: 3.62

Seq Identity: 0.34 | Length: 755 / 873

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000095002	mutS homolog 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	52	40	88
disruptive	10	50	53	123
missense	9	48	53	107

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	126	55	76
disruptive	22	163	87	209
missense	17	145	78	152

PlasmoDB Total SNPs: 28

Non-coding: 0 | Synonymous: 19 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 873 | Molecular Weight (kDa): 101.546

UniProt ID(s): C0H4L8, Q8IAJ9

PDB ID(s): None

Isoelectric Point: 8.34

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000432	DNA mismatch repair protein MutS, C-terminal
InterPro	IPR007696	DNA mismatch repair protein MutS, core
InterPro	IPR011184	DNA mismatch repair Msh2-type
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR032642	DNA mismatch repair protein Msh2
InterPro	IPR036187	DNA mismatch repair protein MutS, core domain superfamily
PFam	PF00488	DNA mismatch repair protein MutS, C-terminal
PFam	PF05192	DNA mismatch repair protein MutS, core
Superfamily	SSF48334	DNA mismatch repair protein MutS, core domain superfamily
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase