Product Description: E3 ubiquitin-protein ligase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0051087 | chaperone binding |
| Function | GO:0061630 | ubiquitin protein ligase activity |
| Process | GO:0071218 | cellular response to misfolded protein |
| Process | GO:0045862 | positive regulation of proteolysis |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0000209 | protein polyubiquitination |
| Process | GO:0006515 | protein quality control for misfolded or incompletely synthesized proteins |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.64 | 0.70 |
| Trophozoite | expressed | 0.75 | 0.59 |
| Schizont | expressed | 1.07 | 0.46 |
| Gametocyte | possibly expressed | 0.88 | 0.54 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0707700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.998 | MFS: -2.479 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0804900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0804900)
Modification: Disrupted | RMgm-2307
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IC12
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q02127 | Dihydroorotate dehydrogenase (quinone), mitochondrial | Homo sapiens | OrthoDB | US8536165, 110tricyclic compound 5TERIFLUNOMIDEBiphenquinate... |
| A0A509AEH5 | Dihydroorotate dehydrogenase (quinone), mitochondrial [100-517] | Plasmodium berghei | OrthoDB | JMC521864 Compund DSM1JMC521864 Compund DSM2cid_741601triazolopyrimidine-based compound, DSM98... |
| Q63707 | Dihydroorotate dehydrogenase (quinone), mitochondrial | Rattus norvegicus | OrthoDB | AtovaquoneBiphenquinateAtovaquoneBiphenquinate... |
| O35435 | Dihydroorotate dehydrogenase (quinone), mitochondrial | Mus musculus | OrthoDB | CHEMBL3289670CHEMBL3289672TERIFLUNOMIDECHEMBL3289671... |
| G4VFD7 | Dihydroorotate dehydrogenase (quinone), mitochondrial | OrthoDB | CHEMBL4436314CHEMBL4560384ATOVAQUONECHEMBL2408377... | |
| Q08210 | Dihydroorotate dehydrogenase (quinone), mitochondrial | Plasmodium falciparum | OrthoDB | tricyclic compound 5tricyclic compound 6bicyclic compound 7monocyclic compound 8... |
Ortholog Group (OrthoMCL): OG6_103776
Most Similar Human Ortholog: Q9UNE7
TM-align score: 0.68 | RMSD: 3.65
Seq Identity: 0.29 | Length: 255 / 961
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000103266 | STIP1 homology and U-box containing protein 1 |
Protein Length: 961 | Molecular Weight (kDa): 110.779
UniProt ID(s): Q8IC12
PDB ID(s): None
Isoelectric Point: 8.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003613 | U box domain |
| InterPro | IPR011990 | Tetratricopeptide-like helical domain superfamily |
| InterPro | IPR019734 | Tetratricopeptide repeat |
| PFam | PF04564 | U box domain |
| Superfamily | SSF48452 | Tetratricopeptide-like helical domain superfamily |
| Superfamily | SSF57850 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 38 | 20 | 43 |
| disruptive | 40 | 70 | 52 | 111 |
| missense | 19 | 50 | 42 | 88 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 106 | 27 | 60 |
| disruptive | 69 | 215 | 81 | 290 |
| missense | 31 | 161 | 64 | 150 |
PlasmoDB Total SNPs: 215
Non-coding: 129 | Synonymous: 51 | Nonsynonymous: 35 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |