PF3D7_0708000

Genome location: Pf3D7_07_v3:368,274..372,081(+)

Genome classification: Core

Function and Localization

Product Description: cytoskeleton associated protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0043014	alpha-tubulin binding
Process	GO:0000226	microtubule cytoskeleton organization
Process	GO:0007023	post-chaperonin tubulin folding pathway
Process	GO:0007021	tubulin complex assembly

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.08	0.96
Trophozoite	expressed	0.35	0.80
Schizont	expressed	0.09	0.95
Gametocyte	expressed	0.60	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0708000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.132 | MFS: -2.842 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0805200)

Relative Growth Rate: 0.08 ± 0.10
Confidence: 5.96

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4M2

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_107210

Most Similar Human Ortholog: E9PNS0

TM-align score: 0.82 | RMSD: 3.04

Seq Identity: 0.22 | Length: 225 / 868

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000154114	tubulin folding cofactor E like
ENSG00000285509	TBCEL-TECTA readthrough

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	35	17	55
disruptive	25	65	41	108
missense	16	50	31	90

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	82	31	57
disruptive	41	140	82	185
missense	24	111	59	125

PlasmoDB Total SNPs: 165

Non-coding: 110 | Synonymous: 45 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 868 | Molecular Weight (kDa): 103.589

UniProt ID(s): C0H4M2

PDB ID(s): None

Isoelectric Point: 6.86

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000938	CAP Gly-rich domain
InterPro	IPR029071	Ubiquitin-like domain superfamily
InterPro	IPR036859	CAP Gly-rich domain superfamily
PFam	PF01302	CAP Gly-rich domain
Superfamily	SSF52047	Ubiquitin-like domain superfamily
Superfamily	SSF54236	CAP Gly-rich domain superfamily
Superfamily	SSF74924