Product Description: DNA-directed RNA polymerases I, II, and III subunit RPABC5, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005736 | RNA polymerase I complex |
| Component | GO:0005665 | RNA polymerase II, core complex |
| Component | GO:0005666 | RNA polymerase III complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003899 | DNA-directed 5'-3' RNA polymerase activity |
| Function | GO:0008270 | zinc ion binding |
| Process | GO:0042797 | tRNA transcription by RNA polymerase III |
| Process | GO:0006360 | transcription by RNA polymerase I |
| Process | GO:0006366 | transcription by RNA polymerase II |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.03 | 0.98 |
| Schizont | expressed | 0.00 | 1.00 |
| Gametocyte | expressed | 0.02 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0708100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.126 | MFS: 0 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IC08
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101548
Most Similar Human Ortholog: P62875
TM-align score: 0.97 | RMSD: 0.55
Seq Identity: 0.55 | Length: 67 / 69
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000177700 | RNA polymerase II, I and III subunit L |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 1 | 1 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 1 |
| disruptive | 1 | 1 | 1 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 66
Non-coding: 64 | Synonymous: 2 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 69 | Molecular Weight (kDa): 8.241
UniProt ID(s): Q8IC08
PDB ID(s): None
Isoelectric Point: 8.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000268 | DNA-directed RNA polymerase, subunit N/Rpb10 |
| InterPro | IPR020789 | RNA polymerases, subunit N, zinc binding site |
| InterPro | IPR023580 | RNA polymerase subunit RPB10 |
| PFam | PF01194 | DNA-directed RNA polymerase, subunit N/Rpb10 |
| Superfamily | SSF46924 | RNA polymerase subunit RPB10 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 14698245 | Heterologous overexpression and purification of four common subunits of nuclearRNA polymerases I, II and III of Schizosaccharomyces pombe | Proshkin SA, Shpakovski GV | 10.1016/j.jchromb.2003.09.008 |
| 20536828 | A screen for genes involved in respiration control and longevity inSchizosaccharomyces pombe | Roux AE, Arseneault G, ..., Rokeach LA | 10.1111/j.1749-6632.2010.05198.x |
| 16621069 | Characterization of RNA polymerase II subunits of Trypanosoma brucei | Devaux S, Lecordier L, ..., Vanhamme L | 10.1016/j.molbiopara.2006.02.020 |
| 15569151 | Hub1 is an essential ubiquitin-like protein without functioning as a typicalmodifier in fission yeast | Yashiroda H, Tanaka K | 10.1111/j.1365-2443.2004.00807.x |
| 16730080 | Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei | Nguyen TN, Schimanski B, ..., Gunzl A | 10.1016/j.molbiopara.2006.02.023 |
| 11168594 | Transcription organization and mRNA levels of the genes for all 12 subunits ofthe fission yeast RNA polymerase II | Sakurai H, Ishihama A | 10.1046/j.1365-2443.2001.00394.x |
| 11918671 | Level of the RNA polymerase II in the fission yeast stays constant butphosphorylation of its carboxyl terminal domain varies depending on the phase andrate of cell growth | Sakurai H, Ishihama A | 10.1046/j.1365-2443.2002.00522.x |
| 11168400 | Intracellular contents and assembly states of all 12 subunits of the RNApolymerase II in the fission yeast Schizosaccharomyces pombe | Kimura M, Sakurai H, Ishihama A | 10.1046/j.1432-1327.2001.01911.x |
| 10653691 | Functional conservation of RNA polymerase II in fission and budding yeasts | Shpakovski GV, Gadal O, ..., Thuriaux P | 10.1006/jmbi.1999.3399 |