Product Description: EKC/KEOPS complex subunit BUD32
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase);2.7.11.2 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000408 | EKC/KEOPS complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0070525 | tRNA threonylcarbamoyladenosine metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.06 | 0.97 |
| Schizont | possibly expressed | 0.01 | 0.99 |
| Gametocyte | not expressed | 0.04 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0708300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.91 | MFS: -3.11 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0805600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IC06
"Best" AlphaFill ligand hit: TBR (dodecabromohexatantalum, Local RMSD=0.18) with 3EN9 (Global RMSD=3.06)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101388
Most Similar Human Ortholog: Q96S44
TM-align score: 0.84 | RMSD: 1.75
Seq Identity: 0.35 | Length: 208 / 229
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000172315 | TP53 regulating kinase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 7 | 8 | 12 |
| disruptive | 2 | 13 | 13 | 23 |
| missense | 2 | 13 | 12 | 19 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 24 | 11 | 15 |
| disruptive | 4 | 49 | 21 | 71 |
| missense | 2 | 43 | 15 | 48 |
PlasmoDB Total SNPs: 164
Non-coding: 159 | Synonymous: 3 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 229 | Molecular Weight (kDa): 26.615
UniProt ID(s): Q8IC06
PDB ID(s): None
Isoelectric Point: 9.92
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR008266 | Tyrosine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR022495 | Serine/threonine-protein kinase Bud32 |
| PFam | N/A | N/A |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 25204654 | Kinase-associated endopeptidase 1 (Kae1) participates in an atypicalribosome-associated complex in the apicoplast of Plasmodium falciparum. | Mallari JP, Oksman A, Vaupel B, Goldberg DE | 10.1074/jbc.M114.586735 |
| 36416748 | Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidaseactivity | Li QX, Liu JC, He MH, Zhou JQ | 10.1042/BCJ20220290 |
| 25735745 | Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a modelfor the complete yeast KEOPS complex | Zhang W, Collinet B, ..., van Tilbeurgh H | 10.1093/nar/gkv155 |
| 27903914 | Proteomic analysis of the human KEOPS complex identifies C14ORF142 as a coresubunit homologous to yeast Gon7 | Wan LC, Maisonneuve P, ..., Sicheri F | 10.1093/nar/gkw1181 |
| 29804714 | Yeast KEOPS complex regulates telomere length independently of its t(6)Amodification function | Liu YY, He MH, ..., Zhou JQ | 10.1016/j.jgg.2018.03.004 |