PF3D7_0708700 (COX4)

Genome location: Pf3D7_07_v3:390,832..392,770(-)

Genome classification: Core

Function and Localization

Product Description: cytochrome c oxidase subunit 4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.04	0.98
Trophozoite	expressed	0.25	0.83
Schizont	expressed	0.20	0.85
Gametocyte	expressed	0.35	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0708700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.994 | MFS: -2.415 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1219200)

Relative Growth Rate: 0.06 ± 0.05
Confidence: 7.50

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IC02

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_139486

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	7	6	12
disruptive	2	10	9	18
missense	2	9	8	14

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	17	9	14
disruptive	2	32	20	39
missense	2	27	17	18

PlasmoDB Total SNPs: 97

Non-coding: 86 | Synonymous: 3 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 228 | Molecular Weight (kDa): 27.55

UniProt ID(s): O15796, Q8IC02

PDB ID(s): None

Isoelectric Point: 6.13

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004203	Cytochrome c oxidase subunit IV family
PFam	N/A	N/A
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
30204084	Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presenceof a divergent cytochrome c oxidase	Seidi A, Muellner-Wong LS, ..., van Dooren GG	10.7554/eLife.38131
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21
37891015	Promoter-proximal introns impact recombinant amylase expression in Saccharomycescerevisiae	Schwerdtfeger KS, Myburgh MW, van Zyl WH, Viljoen-Bloom M	10.1093/femsyr/foad047