PF3D7_0709300 (MED14)

Genome location: Pf3D7_07_v3:413,560..422,436(-)

Genome classification: Core

Function and Localization

Product Description: mediator of RNA polymerase II transcription subunit 14, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0031910	cytostome
Component	GO:0020020	food vacuole
Component	GO:0016592	mediator complex
Component	GO:0005730	nucleolus
Component	GO:0020005	symbiont-containing vacuole membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.55	0.75
Trophozoite	expressed	0.93	0.52
Schizont	expressed	0.23	0.86
Gametocyte	expressed	0.84	0.55

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0709300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 6 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.141 | MFS: -2.91 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156420

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	30	71	46	126
disruptive	119	290	172	424
missense	79	225	148	347

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	46	159	75	141
disruptive	210	566	336	557
missense	113	449	282	417

PlasmoDB Total SNPs: 398

Non-coding: 79 | Synonymous: 164 | Nonsynonymous: 155 | Stop Codon: 0

Protein Information

Protein Length: 2729 | Molecular Weight (kDa): 325.491

UniProt ID(s): O15791, O15792, O15801, Q8IBZ6, Q9BH29, Q9BH64, Q9BHA7, Q9BHS4, Q9BHS5, Q9BHS6, Q9BHS7, Q9BHS8, Q9BHS9, Q9BHT0, Q9BHT1, Q9BHT2, Q9BHT3, Q9BHT4, Q9BHT5, Q9BHT7, Q9GN11, Q9GSR7, Q9GSR9

PDB ID(s): None

Isoelectric Point: 7.79

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013947	Mediator complex, subunit Med14
PFam	PF08638	Mediator complex, subunit Med14
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
16183150	PfCG2, a Plasmodium falciparum protein peripherally associated with theparasitophorous vacuolar membrane, is expressed in the period of maximumhemoglobin uptake and digestion by trophozoites.	Cooper RA, Papakrivos J, ..., Wellems TE	10.1016/j.molbiopara.2005.07.009
35557691	Mediator Complex of the Malaria Parasite Plasmodium falciparum Associates withEvolutionarily Novel Subunits.	Iyer UB, Park JE, ..., Featherstone M	10.1021/acsomega.2c00368
33789343	Architectural Mediator subunits are differentially essential for globaltranscription in Saccharomyces cerevisiae	Tourigny JP, Schumacher K, ..., Zentner GE	10.1093/genetics/iyaa042