PF3D7_0709600 (POP1)

Genome location: Pf3D7_07_v3:429,537..433,997(-)

Genome classification: Core

Function and Localization

Product Description: ribonucleases P/MRP protein subunit POP1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.26.5 (Ribonuclease P)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005655	nucleolar ribonuclease P complex
Component	GO:0000172	ribonuclease MRP complex
Component	GO:0030677	ribonuclease P complex
Function	GO:0000171	ribonuclease MRP activity
Function	GO:0004526	ribonuclease P activity
Process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
Process	GO:0008033	tRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.57	0.73
Trophozoite	expressed	0.51	0.71
Schizont	expressed	0.11	0.93
Gametocyte	not expressed	0.22	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0709600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.123 | MFS: -3.036 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1220100)

Relative Growth Rate: 0.10 ± 0.76
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4M3

"Best" AlphaFill ligand hit: T3F (thymidine-5'-diphosphate-alpha-d-3,6-dideoxy-3-aminogalactose, Local RMSD=3.45) with 7N7B (Global RMSD=18.88)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.26.5	ribonuclease P	ATPRNAEDTANH4+tRNASO42-NeoR5NeoG6NeoK6retinolneaminemat-tRNA...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103108

Most Similar Human Ortholog: Q99575

TM-align score: 0.55 | RMSD: 5.05

Seq Identity: 0.21 | Length: 638 / 1156

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000104356	POP1 homolog, ribonuclease P/MRP subunit

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	21	44	34	62
disruptive	33	115	74	177
missense	29	112	71	154

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	28	92	41	76
disruptive	65	260	118	206
missense	43	228	111	167

PlasmoDB Total SNPs: 198

Non-coding: 97 | Synonymous: 62 | Nonsynonymous: 38 | Stop Codon: 1

Protein Information

Protein Length: 1156 | Molecular Weight (kDa): 138.305

UniProt ID(s): C0H4M3

PDB ID(s): None

Isoelectric Point: 10.38

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR009723	Pop1, N-terminal
InterPro	IPR012590	POPLD domain
InterPro	IPR039182	Ribonucleases P/MRP protein subunit Pop1
PFam	PF06978	Pop1, N-terminal
PFam	PF08170	POPLD domain
Superfamily	N/A	N/A