PF3D7_0710400 (RAD14)

Genome location: Pf3D7_07_v3:474,350..475,968(+)

Genome classification: Core

Function and Localization

Product Description: DNA repair protein RAD14, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 4.2.99.18 (DNA-(apurinic or apyrimidinic site) lyase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000110	nucleotide-excision repair factor 1 complex
Function	GO:0003684	damaged DNA binding
Process	GO:0070914	UV-damage excision repair
Process	GO:0006284	base-excision repair
Process	GO:1901255	nucleotide-excision repair involved in interstrand cross-link repair
Process	GO:0000715	nucleotide-excision repair, DNA damage recognition
Process	GO:0033683	nucleotide-excision repair, DNA incision

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.07	0.96
Trophozoite	not expressed	0.19	0.88
Schizont	possibly expressed	0.06	0.96
Gametocyte	not expressed	0.16	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0710400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.155 | MFS: -2.88 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1221000)

Relative Growth Rate: 1.04 ± 0.10
Confidence: 5.90

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1221000)

Modification: Disrupted | RMgm-3278

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZWE7

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.2.99.18	DNA-(apurinic or apyrimidinic site) lyase	ATPAMPKClCMPUMPP53EDTAdCMPdTMPdAMPdGMPATCA...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104388

Most Similar Human Ortholog: F2Z2T2

TM-align score: 0.42 | RMSD: 3.09

Seq Identity: 0.23 | Length: 128 / 365

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000136936	XPA, DNA damage recognition and repair factor

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	7	5	14
disruptive	13	41	30	69
missense	11	32	28	61

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	14	6	30
disruptive	28	119	50	121
missense	16	98	37	70

PlasmoDB Total SNPs: 72

Non-coding: 43 | Synonymous: 17 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 365 | Molecular Weight (kDa): 43.811

UniProt ID(s): A0A143ZWE7

PDB ID(s): None

Isoelectric Point: 6.35

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000465	XPA
InterPro	IPR009061	Putative DNA-binding domain superfamily
PFam	N/A	N/A
Superfamily	SSF46955	Putative DNA-binding domain superfamily

PMID	Title	Authors	DOI/Link
20421413	Abasic sites in the transcribed strand of yeast DNA are removed bytranscription-coupled nucleotide excision repair	Kim N, Jinks-Robertson S	10.1128/MCB.00308-10
35946552	Diploid-associated adaptation to chronic low-dose UV irradiation requireshomologous recombination in Saccharomyces cerevisiae	Shibata M, Keyamura K, ..., Hishida T	10.1093/genetics/iyac115
38260495	Multiple DNA repair pathways prevent acetaldehyde-induced mutagenesis in yeast	Porcher L, Vijayraghavan S, ..., Saini N	10.1101/2024.01.07.574575
30846712	Green tea extract promotes DNA repair in a yeast model	Chong SY, Chiang HY, ..., Lo YC	10.1038/s41598-019-39082-9
22197481	Formaldehyde-induced mutagenesis in Saccharomyces cerevisiae: molecularproperties and the roles of repair and bypass systems	Grogan D, Jinks-Robertson S	10.1016/j.mrfmmm.2011.12.004
20546858	Rad10-YFP focus induction in response to UV depends on RAD14 in yeast	Mardiros A, Benoun JM, ..., Fischhaber PL	10.1016/j.acthis.2010.03.005