PF3D7_0713400 (SR25)

Genome location: Pf3D7_07_v3:612,059..613,810(+)

Genome classification: Core

Function and Localization

Product Description: GPCR-like receptor SR25

SignalP Peptide: N/A

# Transmembrane Domains: 8

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005886	plasma membrane
Process	GO:0006884	cell volume homeostasis
Process	GO:0006950	response to stress
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.62	0.69
Trophozoite	expressed	0.43	0.74
Schizont	expressed	0.31	0.80
Gametocyte	possibly expressed	0.19	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0713400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -3.149 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1420000)

Relative Growth Rate: 0.16 ± 0.17
Confidence: 4.93

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBW3

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=3.93) with 6VVG (Global RMSD=14.77)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_139504

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	21	9	7
disruptive	3	6	5	20
missense	3	5	5	13

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	32	4	7
disruptive	10	22	26	78
missense	5	14	20	36

PlasmoDB Total SNPs: 74

Non-coding: 59 | Synonymous: 13 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 357 | Molecular Weight (kDa): 43.096

UniProt ID(s): Q8IBW3

PDB ID(s): None

Isoelectric Point: 9.21

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
28842684	Plasmodium falciparum GPCR-like receptor SR25 mediates extracellular K(+) sensingcoupled to Ca(2+) signaling and stress survival.	Moraes MS, Budu A, ..., Garcia CRS	10.1038/s41598-017-09959-8
33790879	The Knockout for G Protein-Coupled Receptor-Like PfSR25 Increases theSusceptibility of Malaria Parasites to the Antimalarials Lumefantrine andPiperaquine but Not to Medicine for Malaria Venture Compounds.	Santos BM, Dias BKM, Nakabashi M, Garcia CRS	10.3389/fmicb.2021.638869
37108438	Changes in K(+) Concentration as a Signaling Mechanism in the ApicomplexaParasites Plasmodium and Toxoplasma	Santos BMD, Przyborski JM, Garcia CRS	10.3390/ijms24087276
32824696	Plasmodium falciparum Knockout for the GPCR-Like PfSR25 Receptor Displays GreaterSusceptibility to 1,2,3-Triazole Compounds That Block Malaria ParasiteDevelopment	Santos BMD, Gonzaga DTG, ..., Garcia CRS	10.3390/biom10081197