PF3D7_0714300 (DHHC4)

Genome location: Pf3D7_07_v3:656,565..662,123(+)

Genome classification: Core

Function and Localization

Product Description: palmitoyltransferase DHHC4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0005783	endoplasmic reticulum
Component	GO:0031211	endoplasmic reticulum palmitoyltransferase complex
Function	GO:0016409	palmitoyltransferase activity
Function	GO:0019706	protein-cysteine S-palmitoyltransferase activity
Process	GO:0018230	peptidyl-L-cysteine S-palmitoylation
Process	GO:0018345	protein palmitoylation
Process	GO:0006612	protein targeting to membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.63	0.70
Trophozoite	expressed	0.63	0.64
Schizont	expressed	0.23	0.85
Gametocyte	expressed	0.85	0.55

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0714300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.299 | #Insertions: 4

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1420900)

Relative Growth Rate: 0.33 ± 0.40
Confidence: 3.23

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1420900)

Modification: Tagged | RMgm-898

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBV4

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.-	Ex: [myelin-proteolipid] O-palmitoyltransferase	CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.225	protein S-acyltransferase	stearoyl-CoAmyristoyl-CoA2-Bromopalmitate2-bromo-palmitateacyl-CoA binding protein

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100625

Most Similar Human Ortholog: E9PJS3

TM-align score: 0.84 | RMSD: 2.02

Seq Identity: 0.41 | Length: 147 / 1321

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000175048	zinc finger DHHC-type palmitoyltransferase 14
ENSG00000188706	zinc finger DHHC-type palmitoyltransferase 9
ENSG00000204160	zinc finger DHHC-type palmitoyltransferase 18

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	48	48	50
disruptive	34	126	89	223
missense	26	99	81	177

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	114	54	77
disruptive	89	325	182	351
missense	45	249	128	222

PlasmoDB Total SNPs: 277

Non-coding: 123 | Synonymous: 105 | Nonsynonymous: 49 | Stop Codon: 0

Protein Information

Protein Length: 1321 | Molecular Weight (kDa): 158.748

UniProt ID(s): Q8IBV4

PDB ID(s): None

Isoelectric Point: 9.72

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001594	Palmitoyltransferase, DHHC domain
PFam	PF01529	Palmitoyltransferase, DHHC domain
Superfamily	N/A	N/A