PF3D7_0715000 (PNPase)

Genome location: Pf3D7_07_v3:673,042..674,476(+)

Genome classification: Core

Function and Localization

Product Description: 4-nitrophenylphosphatase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.3.41 (4-nitrophenylphosphatase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Function	GO:0016791	phosphatase activity
Process	GO:0016311	dephosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.38	0.81
Trophozoite	expressed	0.52	0.70
Schizont	not expressed	0.08	0.95
Gametocyte	not expressed	0.14	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0715000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.913 | MFS: -2.49 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBV0

"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.77) with 2X4D (Global RMSD=4.75)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.3.41	4-nitrophenylphosphatase	ATPOuabainvanadatearsenatephosphateo-vanadateorthovanadateethane diamineEthacrynic acidcholine chlorideFluorescein isothiocyanate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100677

Most Similar Human Ortholog: Q96GD0

TM-align score: 0.87 | RMSD: 2.24

Seq Identity: 0.29 | Length: 279 / 322

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000184207	phosphoglycolate phosphatase
ENSG00000241360	pyridoxal phosphatase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	16	6	18
disruptive	3	32	7	36
missense	3	31	6	36

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	26	7	19
disruptive	11	52	21	54
missense	6	48	18	45

PlasmoDB Total SNPs: 53

Non-coding: 43 | Synonymous: 7 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 322 | Molecular Weight (kDa): 36.823

UniProt ID(s): A5PGW7, Q8IBV0

PDB ID(s): None

Isoelectric Point: 6.69

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006349	2-phosphoglycolate phosphatase, eukaryotic
InterPro	IPR006357	HAD-superfamily hydrolase, subfamily IIA
InterPro	IPR036412	HAD-like superfamily
PFam	PF13242	HAD-superfamily hydrolase, subfamily IIA
PFam	PF13344
Superfamily	SSF56784	HAD-like superfamily