PF3D7_0715300

Genome location: Pf3D7_07_v3:683,130..686,996(-)

Genome classification: Core

Function and Localization

Product Description: calcium/calmodulin-dependent protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.17 (Calcium/calmodulin-dependent protein kinase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	not expressed	0.20	0.87
Schizont	not expressed	0.17	0.88
Gametocyte	not expressed	0.19	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0715300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.977 | MFS: -0.436 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1421600)

Relative Growth Rate: 1.00 ± 0.04
Confidence: 7.60

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1421600)

Modification: Disrupted | RMgm-3844

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZZT8

"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=0.03) with 2VZ6 (Global RMSD=2.27)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.17	Ca2+/calmodulin-dependent protein kinase	NOADPGTPW13AIPEDTAEGTADTNBKN93KN62KN-93KN-62...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_136970

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	23	13	24
disruptive	6	34	11	72
missense	6	33	11	64

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	48	21	35
disruptive	11	66	54	139
missense	10	56	44	101

PlasmoDB Total SNPs: 277

Non-coding: 257 | Synonymous: 14 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 517 | Molecular Weight (kDa): 61.331

UniProt ID(s): A0A143ZZT8

PDB ID(s): None

Isoelectric Point: 7

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
PFam	PF00069	Protein kinase domain
Superfamily	SSF56112	Protein kinase-like domain superfamily