Product Description: cysteine desulfurase
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.8.1.7 (Cysteine desulfurase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0031071 | cysteine desulfurase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006534 | cysteine metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.32 | 0.80 |
| Schizont | expressed | 0.05 | 0.96 |
| Gametocyte | expressed | 0.53 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0716600
Resistome Missense Mutations: I184M
Resistome Compounds with Missense Mutations: Suloctidil
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.408 | MFS: -2.488 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0614300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0614300)
Modification: Disrupted | RMgm-4879
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IBT4
"Best" AlphaFill ligand hit: PLG (n-pyridoxyl-glycine-5-monophosphate, Local RMSD=0.12) with 7TLP (Global RMSD=3.03)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.8.1.7 | cysteine desulfurase | NaBH4K2TeO3L-cysteineVinylglycineiodoacetamideD-cycloserinedithiothreitolL-AllylglycineN-ethylmaleimideL-selenocysteineinterferon-gammalipopolysaccharide... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 20 | 13 | 21 |
| disruptive | 8 | 35 | 16 | 50 |
| missense | 7 | 33 | 14 | 47 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 44 | 8 | 34 |
| disruptive | 12 | 77 | 44 | 99 |
| missense | 10 | 62 | 34 | 77 |
PlasmoDB Total SNPs: 152
Non-coding: 122 | Synonymous: 23 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 546 | Molecular Weight (kDa): 64.561
UniProt ID(s): Q8IBT4
PDB ID(s): None
Isoelectric Point: 9.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000192 | Aminotransferase class V domain |
| InterPro | IPR015424 | Pyridoxal phosphate-dependent transferase |
| PFam | PF00266 | Aminotransferase class V domain |
| Superfamily | SSF53383 | Pyridoxal phosphate-dependent transferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 24086138 | The suf iron-sulfur cluster synthesis pathway is required for apicoplastmaintenance in malaria parasites. | Gisselberg JE, Dellibovi-Ragheb TA, ..., Prigge ST | 10.1371/journal.ppat.1003655 |
| 24709262 | Sulfur mobilization for Fe-S cluster assembly by the essential SUF pathway in thePlasmodium falciparum apicoplast and its inhibition. | Charan M, Singh N, ..., Habib S | 10.1128/AAC.02711-13 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 32133993 | Structural evidence for a latch mechanism regulating access to the active site ofSufS-family cysteine desulfurases | Dunkle JA, Bruno MR, Frantom PA | 10.1107/S2059798320000790 |
| 32822728 | Fe-S cluster biogenesis by the bacterial Suf pathway | Blahut M, Sanchez E, Fisher CE, Outten FW | 10.1016/j.bbamcr.2020.118829 |
| 33320434 | The sulfur formation system mediating extracellular cysteine-cystine recycling inFervidobacterium islandicum AW-1 is associated with keratin degradation | Jin HS, Dhanasingh I, ..., Lee DW | 10.1111/1751-7915.13717 |
| 37166116 | The Plasmodium falciparum apicoplast cysteine desulfurase provides sulfur forboth iron-sulfur cluster assembly and tRNA modification | Swift RP, Elahi R, ..., Prigge ST | 10.7554/eLife.84491 |
| 37238602 | Structural and Biochemical Characterization of Mycobacterium tuberculosis ZincSufU-SufS Complex | Elchennawi I, Carpentier P, ..., Ollagnier de Choudens S | 10.3390/biom13050732 |
| 36506149 | Structural and Biochemical Characterization of Staphylococcus aureus CysteineDesulfurase Complex SufSU | Hudspeth JD, Boncella AE, ..., Morrison CN | 10.1021/acsomega.2c05576 |
| 35395703 | Cycloserine enantiomers inhibit PLP-dependent cysteine desulfurase SufS viadistinct mechanisms | Nakamura R, Ogawa S, Takahashi Y, Fujishiro T | 10.1111/febs.16455 |
| 35377584 | Structural diversity of cysteine desulfurases involved in iron-sulfur cluster biosynthesis | Fujishiro T, Nakamura R, Kunichika K, Takahashi Y | 10.2142/biophysico.bppb-v19.0001 |
| 32184761 | Iron-Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria | Gao F | 10.3389/fmicb.2020.00165 |
| 35467390 | Sulfur Availability Impacts Accumulation of the 2-Thiouridine tRNA Modificationin Bacillus subtilis | Edwards AM, Black KA, Dos Santos PC | 10.1128/jb.00009-22 |
| 35427399 | Mycobacterium tuberculosis requires SufT for Fe-S cluster maturation, metabolism,and survival in vivo | Tripathi A, Anand K, ..., Singh A | 10.1371/journal.ppat.1010475 |