PF3D7_0717300

Genome location: Pf3D7_07_v3:747,860..751,189(+)

Genome classification: Core

Function and Localization

Product Description: transcription initiation factor IIE subunit alpha, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005673	transcription factor TFIIE complex
Function	GO:0000993	RNA polymerase II complex binding
Process	GO:0006366	transcription by RNA polymerase II
Process	GO:0006367	transcription initiation from RNA polymerase II promoter
Process	GO:0001113	transcription open complex formation at RNA polymerase II promoter

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.48	0.77
Trophozoite	expressed	0.53	0.70
Schizont	expressed	0.52	0.70
Gametocyte	expressed	0.93	0.52

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0717300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.486 | MFS: -2.701 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0615000)

Relative Growth Rate: 0.28 ± 0.30
Confidence: 3.81

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4N3

"Best" AlphaFill ligand hit: P52 (nalpha-[(2s)-2-{[[(1r)-1-amino-3-phenylpropyl](hydroxy)phosphoryl]methyl}-4-methylpentanoyl]-l-tryptophanamide, Local RMSD=0.60) with 4JBS (Global RMSD=17.44)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_124342

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	14	11	24
disruptive	4	25	11	31
missense	4	22	11	25

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	32	14	36
disruptive	13	53	26	73
missense	8	41	21	51

PlasmoDB Total SNPs: 215

Non-coding: 201 | Synonymous: 8 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 392 | Molecular Weight (kDa): 46.825

UniProt ID(s): C0H4N3

PDB ID(s): None

Isoelectric Point: 6.64

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR024550	TFIIEalpha/SarR/Rpc3 HTH domain
InterPro	IPR039997	Transcription factor E
PFam	PF02002	TFIIEalpha/SarR/Rpc3 HTH domain
Superfamily	N/A	N/A