PF3D7_0717400

Genome location: Pf3D7_07_v3:751,584..755,027(+)

Genome classification: Core

Function and Localization

Product Description: queuine tRNA-ribosyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.4.2.29 (tRNA-guanine(34) transglycosylase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0008479	queuine tRNA-ribosyltransferase activity
Function	GO:0008270	zinc ion binding
Process	GO:0008616	queuosine biosynthetic process
Process	GO:0006400	tRNA modification
Process	GO:0002099	tRNA wobble guanine modification

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.43	0.79
Trophozoite	expressed	0.45	0.75
Schizont	expressed	0.29	0.81
Gametocyte	expressed	0.21	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0717400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 1.159 | #Insertions: 5

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0615100)

Relative Growth Rate: 0.87 ± 0.23
Confidence: 4.30

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0615100)

Modification: Disrupted | RMgm-2124

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBS6

"Best" AlphaFill ligand hit: TEW (6-tungstotellurate(vi), Local RMSD=0.20) with 7OVS (Global RMSD=1.97)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.4.2.29	tRNA-guanosine34 preQ1 transglycosylase	ethylacetimidate4-aminophthalimidedimethylsuberimidate4-aminophthalhydrazide3,5-diaminophthalimide2,3-Dihydroxybenzoic acid2-aminopteridin-4(3H)-one3,5-diaminophthalhydrazide2-aminoquinazolin-4(3H)-onebenzimidazole-5-carboxylate2-methylamino-lin-benzoguanine2,6-diaminoquinazolin-4(3H)-one...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9BXR0	Queuine tRNA-ribosyltransferase catalytic subunit 1	Homo sapiens	OrthoDB	CHEMBL6993CHEMBL487739

Orthology Information

Ortholog Group (OrthoMCL): OG6_100602

Most Similar Human Ortholog: K7EL95

TM-align score: 0.94 | RMSD: 1.31

Seq Identity: 0.33 | Length: 136 / 751

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000213339	queuine tRNA-ribosyltransferase catalytic subunit 1

Protein Information

Protein Length: 751 | Molecular Weight (kDa): 88.035

UniProt ID(s): Q8IBS6

PDB ID(s): None

Isoelectric Point: 7.37

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002616	tRNA-guanine(15) transglycosylase-like
InterPro	IPR036511	Queuine tRNA-ribosyltransferase-like
PFam	PF01702	tRNA-guanine(15) transglycosylase-like
Superfamily	SSF51713	Queuine tRNA-ribosyltransferase-like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	25	18	43
disruptive	82	102	50	129
missense	38	55	38	100

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	50	29	54
disruptive	103	190	62	195
missense	46	130	44	130

PlasmoDB Total SNPs: 210

Non-coding: 73 | Synonymous: 53 | Nonsynonymous: 84 | Stop Codon: 0